武汉科技大学学报（自然科学版）
武漢科技大學學報（自然科學版）
무한과기대학학보（자연과학판）
JOURNAL OF WUHAN UNIVERSITY OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCE EDITION)
2014年
6期
458-462
,共5页
苯酚降解菌%生物降解%降解动力学%降解特性%微球菌属
苯酚降解菌%生物降解%降解動力學%降解特性%微毬菌屬
분분강해균%생물강해%강해동역학%강해특성%미구균속
phenol degrading bacterial strain%biodegradation%degradation kinetics%degradation char-acteristic%M icrococcus sp
从活性污泥中经定向驯化、分离纯化得到一株能以苯酚为唯一碳源生长的降解菌P1，通过革兰氏染色和一系列生理生化实验，初步鉴定其为微球菌属。研究菌株接种量、培养基初始pH值、培养温度、摇床转速、金属离子等因素对菌株P1的苯酚降解特性的影响。结果表明，苯酚降解适宜条件为：初始p H值7．0、温度35℃、转速150 r／m in、接种量3％，在此培养条件下，菌株P1可将500 m g／L的苯酚于12 h内完全降解；当苯酚的初始浓度为100～500 m g／L时，菌株P1对苯酚的降解满足M o no d零级反应动力学模型。
從活性汙泥中經定嚮馴化、分離純化得到一株能以苯酚為唯一碳源生長的降解菌P1，通過革蘭氏染色和一繫列生理生化實驗，初步鑒定其為微毬菌屬。研究菌株接種量、培養基初始pH值、培養溫度、搖床轉速、金屬離子等因素對菌株P1的苯酚降解特性的影響。結果錶明，苯酚降解適宜條件為：初始p H值7．0、溫度35℃、轉速150 r／m in、接種量3％，在此培養條件下，菌株P1可將500 m g／L的苯酚于12 h內完全降解；噹苯酚的初始濃度為100～500 m g／L時，菌株P1對苯酚的降解滿足M o no d零級反應動力學模型。
종활성오니중경정향순화、분리순화득도일주능이분분위유일탄원생장적강해균P1，통과혁란씨염색화일계렬생리생화실험，초보감정기위미구균속。연구균주접충량、배양기초시pH치、배양온도、요상전속、금속리자등인소대균주P1적분분강해특성적영향。결과표명，분분강해괄의조건위：초시p H치7．0、온도35℃、전속150 r／m in、접충량3％，재차배양조건하，균주P1가장500 m g／L적분분우12 h내완전강해；당분분적초시농도위100～500 m g／L시，균주P1대분분적강해만족M o no d령급반응동역학모형。
A phenol‐degrading bacterial strain P1 that takes phenol as the sole carbon source was isola‐ted from active sludge through acclimatization method and was preliminarily identified as Micrococcus . The degradation characteristics of P1 were investigated based on such factors as pH value ,tempera‐ture ,inoculum size ,metal ion ,and revolving speed .The results show that the suitable condition of phenol degradation is 35 ℃ ,pH 7 .0 ,inoculum size 3% ,and revolving speed 150 r/min .When the initial concentration of phenol ranges from 100 to 500 mg/L ,the degradation of the strain P1 is found to be in line with Monod zero order reaction kinetics model .
