CAJ | 학술논문

为了解自主跑轮运动对C57BL/6小鼠空间学习记忆能力及海马内Notch信号通路的影响，选用12只雄性5周龄C57BL/6小鼠，随机分为运动组(R组，n=6只)和对照组(C组，n=6只)，运动组进行8周的自主跑轮运动，对照组安静饲养，第8周开始利用Morris水迷宫测试其学习记忆能力。运动结束后，断颈处死小鼠取双侧海马，用Western Blot检测小鼠大脑海马内NICD的蛋白表达水平，用RT-PCR检测小鼠大脑海马内的Jagged-1、Notch-1、PS-1、Hes-1的mRNA表达水平。结果显示：Morris水迷宫实验显示，运动组小鼠学习记忆能力明显强于对照组；Western Blot 测试，运动组小鼠海马内 NICD 表达显著上调，RT-PCR 测试，运动组小鼠海马内 Jagged-1 mRNA、Notch-1 mRNA、PS-1 mRNA和Hes-1 mRNA表达水平较对照组显著上调。结果说明，8周自主跑轮运动激活Notch信号通路，提高了海马空间学习记忆能力。
위료해자주포륜운동대C57BL/6소서공간학습기억능력급해마내Notch신호통로적영향，선용12지웅성5주령C57BL/6소서，수궤분위운동조(R조，n=6지)화대조조(C조，n=6지)，운동조진행8주적자주포륜운동，대조조안정사양，제8주개시이용Morris수미궁측시기학습기억능력。운동결속후，단경처사소서취쌍측해마，용Western Blot검측소서대뇌해마내NICD적단백표체수평，용RT-PCR검측소서대뇌해마내적Jagged-1、Notch-1、PS-1、Hes-1적mRNA표체수평。결과현시：Morris수미궁실험현시，운동조소서학습기억능력명현강우대조조；Western Blot 측시，운동조소서해마내 NICD 표체현저상조，RT-PCR 측시，운동조소서해마내 Jagged-1 mRNA、Notch-1 mRNA、PS-1 mRNA화Hes-1 mRNA표체수평교대조조현저상조。결과설명，8주자주포륜운동격활Notch신호통로，제고료해마공간학습기억능력。
In order to understand the effects of voluntary wheel exercise on spatial learning and memory and Notch signal pathway in hippocampus of C57BL/6 mice, the authors prepared 12 5-week old male C57BL/6 mice, ran-domly divided them into an exercise group (group R, n=6) and a control group (group C, n=6), let the mice in group R do a voluntary wheel exercise for 8 weeks, let the mice in group C live in a calm condition, starting from week 8, tested their learning and memory ability by utilizing Morris water maze, after the exercise was finished, cut the neck of the mice and took out hippocampus at both sides, measured the protein expression level of NICD in hippocampus of the mice by means of Western blot, measured the mRNA expression levels of Jagged-1, Notch-1, PS-1 and Hes-1 in hippocampus in the brains of the mice by means of RT-PCR, and revealed the following findings:the Morris water maze experiment showed that the learning and memory ability of the mice in group R was signifi-cantly stronger than that of the mice in group C;in Western blot test, the expression of NICD in hippocampus of the mice in group R increased significantly;in RT-PCR test, the mRNA expression levels of Jagged-1, Notch-1, PS-1 and Hes-1 in hippocampus of the mice in group R increased significantly as compared with those of the mice in group C. The said findings indicated that 8-week voluntary wheel exercise activated Notch signal pathway, en-hanced the spatial learning and memory ability of hippocampus.