CAJ | 학술논문

设计合成一种全新的苯硫酚检测探针。以试卤灵钠为主要原料，在三乙胺催化下与2，4-二硝基氯苯合成了7-(2’，4’-二硝基苯氧基)-3H-吩噁嗪-3-酮，产率为41.7%。在 pH 7.4的磷酸盐缓冲溶液中，该化合物能与苯硫酚发生亲核取代反应，反应体系在可见光区λ=580 nm 处有强吸收。该探针测定苯硫酚的线性范围为0.1～20μmol／L，检出限为8.9×10–8 mol／L，精密度为3.7%(n=8)，回收率为106%。该方法可快速检测环境及生物体中的苯硫酚。
설계합성일충전신적분류분검측탐침。이시서령납위주요원료，재삼을알최화하여2，4-이초기록분합성료7-(2’，4’-이초기분양기)-3H-분오진-3-동，산솔위41.7%。재 pH 7.4적린산염완충용액중，해화합물능여분류분발생친핵취대반응，반응체계재가견광구λ=580 nm 처유강흡수。해탐침측정분류분적선성범위위0.1～20μmol／L，검출한위8.9×10–8 mol／L，정밀도위3.7%(n=8)，회수솔위106%。해방법가쾌속검측배경급생물체중적분류분。
A kind of brand-new thiophenoldetection probe was designed and synthesised. 7-Hydroxy-3H-phenoxazin-3-one sodium salt as main materials,catalyzed in triethylamine, a new chemodosimeter 7-(2’,4’-dinitrophenoxy)-3H-phenoxazin-3-one was synthetized to determine thiophenol,the yield was 41.7%. It showed that in pH 7.4 phosphate buffer solution,the compound could be reacted with thiophenol,the reaction system had a strong absorption in the visible region of 580 nm,the absorbance increase was linearly with thiophenol concentration from 0.1 μmol/L to 20 μmol/L with a detection limit of 8.9×10–8 mol/L. The precision was 3.7%(n=8),and the recovery rate was 106%. This method provides an effective means for the detection of environmentand organisms of thiophenol.