华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2014年
6期
601-605
,共5页
刘奕%费伟%王丽娜%董广艳%吴红崑
劉奕%費偉%王麗娜%董廣豔%吳紅崑
류혁%비위%왕려나%동엄염%오홍곤
十肽%抗菌性能%口腔感染性疾病
十肽%抗菌性能%口腔感染性疾病
십태%항균성능%구강감염성질병
decapeptide%antibacterial activity%oral infectious disease
目的：??评价人工合成抗菌肽（十肽）对口腔常见感染性疾病主要致病菌的抑菌活性。方法??采用琼脂扩散法及液体稀释法体外评价十肽对变异链球菌、表兄链球菌、嗜酸乳杆菌、血链球菌、格氏链球菌、黏性放线菌、内氏放线菌、牙龈卟啉单胞菌、中间普雷沃菌、具核梭杆菌、伴放线嗜血杆菌及白色假丝酵母菌的抑菌性能，并测定十肽对变异链球菌的时间-杀菌曲线。结果??十肽对所选实验菌株均表现出不同的抑菌性能，对主要致龋菌的最小抑菌浓度MIC值为62.5~125μg·mL-1，而对主要牙周致病菌的MIC值为250~1000μg·mL-1，其中，十肽对龋病主要致病菌变异链球菌有较强抑菌作用。时间-杀菌曲线结果显示，十肽作用20?min后开始杀菌，30?min之后可完全杀灭细菌，且在24?h之内无细菌生长。结论??新型人工合成抗菌肽十肽对口腔常见感染性疾病主要致病菌具有抑菌性能，其中对致龋变异链球菌的抑菌效果最为明显。
目的：??評價人工閤成抗菌肽（十肽）對口腔常見感染性疾病主要緻病菌的抑菌活性。方法??採用瓊脂擴散法及液體稀釋法體外評價十肽對變異鏈毬菌、錶兄鏈毬菌、嗜痠乳桿菌、血鏈毬菌、格氏鏈毬菌、黏性放線菌、內氏放線菌、牙齦卟啉單胞菌、中間普雷沃菌、具覈梭桿菌、伴放線嗜血桿菌及白色假絲酵母菌的抑菌性能，併測定十肽對變異鏈毬菌的時間-殺菌麯線。結果??十肽對所選實驗菌株均錶現齣不同的抑菌性能，對主要緻齲菌的最小抑菌濃度MIC值為62.5~125μg·mL-1，而對主要牙週緻病菌的MIC值為250~1000μg·mL-1，其中，十肽對齲病主要緻病菌變異鏈毬菌有較彊抑菌作用。時間-殺菌麯線結果顯示，十肽作用20?min後開始殺菌，30?min之後可完全殺滅細菌，且在24?h之內無細菌生長。結論??新型人工閤成抗菌肽十肽對口腔常見感染性疾病主要緻病菌具有抑菌性能，其中對緻齲變異鏈毬菌的抑菌效果最為明顯。
목적：??평개인공합성항균태（십태）대구강상견감염성질병주요치병균적억균활성。방법??채용경지확산법급액체희석법체외평개십태대변이련구균、표형련구균、기산유간균、혈련구균、격씨련구균、점성방선균、내씨방선균、아간계람단포균、중간보뢰옥균、구핵사간균、반방선기혈간균급백색가사효모균적억균성능，병측정십태대변이련구균적시간-살균곡선。결과??십태대소선실험균주균표현출불동적억균성능，대주요치우균적최소억균농도MIC치위62.5~125μg·mL-1，이대주요아주치병균적MIC치위250~1000μg·mL-1，기중，십태대우병주요치병균변이련구균유교강억균작용。시간-살균곡선결과현시，십태작용20?min후개시살균，30?min지후가완전살멸세균，차재24?h지내무세균생장。결론??신형인공합성항균태십태대구강상견감염성질병주요치병균구유억균성능，기중대치우변이련구균적억균효과최위명현。
Objective This study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. Methods In this study, we investigated the antimicrobial activity of decapeptide against Streptococcus mutans (S.mutans), Streptococcus sobrinus, Lactobacillus acidophilus, Streptococcus sanguis, Streptococcus gordonii, Actinomyces viscosus, Actinomyces naeslundii, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Saccharomyces albicans in vitro using the agar diffusion method and broth dilution method. Furthermore, a time–kill kinetic study of decapeptide against S. mutans was performed. Results The results showed that decapeptide exhibited antimicrobial activity against various oral bacteria and fungi. The minimum inhibitory concentration (MIC) of main cariogenic bacteria ranged from 62.5 μg·mL-1 to 125 μg·mL-1, and the MIC of periodontopathogenic bacteria tested ranged from 250 μg·mL-1 to 1 000 μg·mL-1. Among the bacteria tested, decapeptide had a strong inhibitory effect on cariogenic S.mutans. Results of the time–kill kinetic studies showed that decapeptide reduced the viable counts of S. mutans by more than one order of magnitude after 20 min of incubation, and thoroughly killed S.mutans after 30 min. No viable cells could be detected after 24 h of incubation. Conclusion This study suggest that decapeptide might have potential clinical application in treating dental caries by killing S.mutans within dental plaque.
