CAJ | 학술논문

在pH8.5的T ris－HCl缓冲液中，曙红Y －中性红和Cu（Ⅱ）相互作用，在538nm处随着Cu（Ⅱ）浓度的增加，体系同步荧光强度逐渐降低，同步荧光强度降低值和Cu（Ⅱ）含量在一定范围内有相关关系。据此应用中性红－曙红Y同步荧光猝灭法建立一种测定铜的新方法。在优化的实验条件下，体系在538nm处的荧光强度降低值与铜的摩尔浓度在7.61×10－8－3.60×10－6 mol · L －1范围内线性关系良好，其线性回归方程为：△ F＝－0.414＋314.89c（×10－6 mol · L －1），相关系数 r＝0.998，检出限为2.48×10－8 mol · L －1，相对标准偏差是0.26％，加标回收率是101.12％。本方法操作简便、灵敏、选择性好，可用于实际样品中铜的测定。
재pH8.5적T ris－HCl완충액중，서홍Y －중성홍화Cu（Ⅱ）상호작용，재538nm처수착Cu（Ⅱ）농도적증가，체계동보형광강도축점강저，동보형광강도강저치화Cu（Ⅱ）함량재일정범위내유상관관계。거차응용중성홍－서홍Y동보형광졸멸법건립일충측정동적신방법。재우화적실험조건하，체계재538nm처적형광강도강저치여동적마이농도재7.61×10－8－3.60×10－6 mol · L －1범위내선성관계량호，기선성회귀방정위：△ F＝－0.414＋314.89c（×10－6 mol · L －1），상관계수 r＝0.998，검출한위2.48×10－8 mol · L －1，상대표준편차시0.26％，가표회수솔시101.12％。본방법조작간편、령민、선택성호，가용우실제양품중동적측정。
In the Tris‐HCl buffer solution of pH8.5 ,the system synchronous fluorescence intensity was obviously decreased with the increace of the concentration of Cu(Ⅱ ) at 538nm due to the interaction between the eosine Y‐neutral red complex and Cu(Ⅱ) ,and the decreased synchronous fluorescence inten‐sity was proportional to the concentration of Cu(Ⅱ) .Based on this phenomenon ,a novel method for the determination of copper was developed .Under the optimal experimental conditions ,there was a linear re‐lationship between the decreased synchronous fluorescence intensity and the concentration of the Copper at 538nm in the range of 7.61 × 10-8‐3.60 × 10-6 mol · L -1 ,The linear regression equation was △ F= -0.414+314.89c ( × 10-6 mol · L -1 ) with a correlation coefficient 0.998 ,and the detection limit was 2.48 × 10-8 mol · L -1 .The relative standard deviation was 0.26% ,and the average recovery was 101.12% . This method is simple ,sensitive and selective ,and has been used successfully in the determination of cop‐per in w ater samples .