CAJ | 학술논문

目的：探讨从人宫颈癌细胞系CaSki中分离培养宫颈癌干细胞球样细胞的可行性，并观察其体外生物学特性。方法采用肿瘤干细胞无血清悬浮培养法富集CaSki细胞系球样细胞，通过体外传代培养、添加血清诱导分化等技术鉴定其干性，利用流式细胞技术检测细胞周期，并应用蛋白印记法及细胞免疫荧光等方法从分子水平检测相关因子的表达。结果从CaSki细胞系中分离到的干细胞球样细胞可以在无血清培养基中悬浮生长成细胞球，并可在体外稳定传代，具有自我更新能力和分化潜能；肿瘤干细胞球样细胞多处于G0/G1期。与其他分子标志物相比，干细胞球样细胞的CD44明显高表达；与亲本细胞相比，干细胞球样细胞的干性相关因子SOX2、OCT4、β-catenin表达较高。结论利用肿瘤干细胞无血清悬浮培养法可以从CaSki细胞系中分离富集到具有稳定肿瘤干细胞性质的细胞群。
목적：탐토종인궁경암세포계CaSki중분리배양궁경암간세포구양세포적가행성，병관찰기체외생물학특성。방법채용종류간세포무혈청현부배양법부집CaSki세포계구양세포，통과체외전대배양、첨가혈청유도분화등기술감정기간성，이용류식세포기술검측세포주기，병응용단백인기법급세포면역형광등방법종분자수평검측상관인자적표체。결과종CaSki세포계중분리도적간세포구양세포가이재무혈청배양기중현부생장성세포구，병가재체외은정전대，구유자아경신능력화분화잠능；종류간세포구양세포다처우G0/G1기。여기타분자표지물상비，간세포구양세포적CD44명현고표체；여친본세포상비，간세포구양세포적간성상관인자SOX2、OCT4、β-catenin표체교고。결론이용종류간세포무혈청현부배양법가이종CaSki세포계중분리부집도구유은정종류간세포성질적세포군。
Objective To isolate and identify sphere-forming stem-like cells from cervical cancer cell line (CaS-ki), and observe its biological characteristics in vitro. Method We enriched sphere-forming stem-like cells using sphere suspension culture system, and confirmed its stem cell-associated characteristics by evaluating the renewal and serum-induced differentiation abilities. Western Blot technology was applied to detect the expression of stem cell-related factors at the protein level. Result The sphere-forming stem-like cells isolated from CaSki can grow into spheres in suspension in serum-free medium, and were able to self-renew. CaSki cancer stem cell-like cells were en-riched in the G0/G1 phase. Compared with other stem cell markers, CD44 has a high expression and can be as the useful marker for cervical cancer cells. Compared with its parental cells, SOX2, OCT4, β -catenin has a high ex-pression of stem cell-related factors. Conclusion We successfully isolated and enriched sphere-forming stem-like cells from cervical cancer cell line CaSki, using serum-free sphere suspension culture system.