广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2014年
24期
116-117,161
,共3页
猪殃殃%绿原酸%高效液相色谱法
豬殃殃%綠原痠%高效液相色譜法
저앙앙%록원산%고효액상색보법
Cleaver%chlorogenic acid%HPLC
建立了猪殃殃中绿原酸含量的HPLC测定方法。色谱柱为Diamonsil C185μm(250 mm×4.6 mm)色谱柱；流动相为甲醇-0.04％磷酸(23：77)；检测波长为324 nm；流速为1 mL/min；进样量为20μL；柱温25℃。经方法学考察，该方法线性关系、精密度、重现性和稳定性好。可快速、准确用于猪殃殃中绿原酸的含量测定。
建立瞭豬殃殃中綠原痠含量的HPLC測定方法。色譜柱為Diamonsil C185μm(250 mm×4.6 mm)色譜柱；流動相為甲醇-0.04％燐痠(23：77)；檢測波長為324 nm；流速為1 mL/min；進樣量為20μL；柱溫25℃。經方法學攷察，該方法線性關繫、精密度、重現性和穩定性好。可快速、準確用于豬殃殃中綠原痠的含量測定。
건립료저앙앙중록원산함량적HPLC측정방법。색보주위Diamonsil C185μm(250 mm×4.6 mm)색보주；류동상위갑순-0.04％린산(23：77)；검측파장위324 nm；류속위1 mL/min；진양량위20μL；주온25℃。경방법학고찰，해방법선성관계、정밀도、중현성화은정성호。가쾌속、준학용우저앙앙중록원산적함량측정。
The HPLC method for determination the content of chlorogenic acid in cleavers was established. The column was Diamonsil C18 5 μm (250 mm×4. 6 mm), the mobile phase was methanol -0. 04%phosphoric acid (23:77 ) , the detection wavelength was 324 nm, the flow rate was 1 mL/min, and the column temperature was 25℃. Through the methodology study, it had the characteristics of the linear relation of the method, precision, reproducibility and stability, which can be used identified Cleavers chlorogenic acid quickly and accurately.
