广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2014年
24期
49-53
,共5页
异养硝化-好氧反硝化菌%培养条件优化%磷酸盐还原
異養硝化-好氧反硝化菌%培養條件優化%燐痠鹽還原
이양초화-호양반초화균%배양조건우화%린산염환원
heterotrophic nitrification-aerobic denitrification%strain optimizing%the reduction of phosphate
通过L9(34)正交试验和单因素实验优化培养条件,从七株不同菌属的异养硝化-好氧反硝化菌中优选兼具除磷性能菌株。结果表明:优选出的两株异养硝化-好氧反硝化菌分别为丛毛单胞菌WXZ-17和芽孢杆菌WXZ2-4,二者均具有厌氧除磷性能,磷化氢为重要除磷产物。优选的两株菌培养条件均为: T=32.0℃、 pH=6.50、氮源为蛋白胨+氯化铵+硝酸钠、总磷浓度为20.0 mg/L,相应的除磷率分别为25.6%和36.0%。在早期脱氮实验中已证实该两株菌脱氮效率分别为:89.7%和96.4%。筛选出的兼具脱氮和除磷性能的菌株使实现废水同步脱氮除磷成为可能。
通過L9(34)正交試驗和單因素實驗優化培養條件,從七株不同菌屬的異養硝化-好氧反硝化菌中優選兼具除燐性能菌株。結果錶明:優選齣的兩株異養硝化-好氧反硝化菌分彆為叢毛單胞菌WXZ-17和芽孢桿菌WXZ2-4,二者均具有厭氧除燐性能,燐化氫為重要除燐產物。優選的兩株菌培養條件均為: T=32.0℃、 pH=6.50、氮源為蛋白胨+氯化銨+硝痠鈉、總燐濃度為20.0 mg/L,相應的除燐率分彆為25.6%和36.0%。在早期脫氮實驗中已證實該兩株菌脫氮效率分彆為:89.7%和96.4%。篩選齣的兼具脫氮和除燐性能的菌株使實現廢水同步脫氮除燐成為可能。
통과L9(34)정교시험화단인소실험우화배양조건,종칠주불동균속적이양초화-호양반초화균중우선겸구제린성능균주。결과표명:우선출적량주이양초화-호양반초화균분별위총모단포균WXZ-17화아포간균WXZ2-4,이자균구유염양제린성능,린화경위중요제린산물。우선적량주균배양조건균위: T=32.0℃、 pH=6.50、담원위단백동+록화안+초산납、총린농도위20.0 mg/L,상응적제린솔분별위25.6%화36.0%。재조기탈담실험중이증실해량주균탈담효솔분별위:89.7%화96.4%。사선출적겸구탈담화제린성능적균주사실현폐수동보탈담제린성위가능。
By orthogonal test and single factor experiments to optimize culture conditions, both phosphorus removal performance was optimized from seven different strains of bacteria with heterotrophic nitrification-aerobic denitrifying. Results showed the optimization of the two heterotrophic nitrification-aerobic denitrifying bacteria of WXZ-17 and WXZ2-4 had the anaerobic phosphorus removal performance and phosphine was the important phosphorus products. Optimization of two bacteria culture conditions were T=32. 0 ℃, pH=6. 50, nitrogen source was peptone and NH4 Cl, NaNO3 , total phosphorus concentration of 20. 0 mg/L, the corresponding phosphorus removal rate were respectively 25. 6% and 36. 0%. In the early denitrification experiments confirmed the two bacteria denitrification efficiency of 89. 7% and 96. 4%, respectively. The strain of denitrification and phosphorus removal performance was screened, and the simultaneous nitrogen and phosphorus wastewater were realized.