CAJ | 학술논문

用制备液相色谱仪(Shimadzu LC-20AP)制备了大豆苷、黄豆黄苷、染料木苷、大豆素、黄豆黄素、染料木素6种大豆异黄酮单体溶液,对其进行了1个月的短期稳定性和6个月的长期稳定性研究,特性量值为溶液的浓度值和纯度值。对在4个不同温度(–20℃、4℃、25℃、60℃)下保存一个月后的测定值与初始值进行了方差分析,短期稳定性试验结果表明,除染料木苷外,其余5种色谱纯大豆异黄酮溶液的浓度在4℃、25℃条件下保存无显著变化,6种大豆异黄酮溶液的纯度在–20℃、4℃、25℃条件下保存无显著变化,因此认为4℃、25℃是大豆异黄酮溶液短期储存的最适温度。在4℃条件下,对经过0、1、2、3、4、6、8、12、16和24周的大豆异黄酮测定值与初始值进行t检验,长期稳定性结果表明,6个月的保存期内6种色谱纯大豆异黄酮溶液的纯度无显著性变化,但浓度均显著增加,分析认为分装容器的密封性是导致浓度增加的原因。本研究结果为大豆异黄酮单体溶液标准溶液产业化提供了技术支撑。
용제비액상색보의(Shimadzu LC-20AP)제비료대두감、황두황감、염료목감、대두소、황두황소、염료목소6충대두이황동단체용액,대기진행료1개월적단기은정성화6개월적장기은정성연구,특성량치위용액적농도치화순도치。대재4개불동온도(–20℃、4℃、25℃、60℃)하보존일개월후적측정치여초시치진행료방차분석,단기은정성시험결과표명,제염료목감외,기여5충색보순대두이황동용액적농도재4℃、25℃조건하보존무현저변화,6충대두이황동용액적순도재–20℃、4℃、25℃조건하보존무현저변화,인차인위4℃、25℃시대두이황동용액단기저존적최괄온도。재4℃조건하,대경과0、1、2、3、4、6、8、12、16화24주적대두이황동측정치여초시치진행t검험,장기은정성결과표명,6개월적보존기내6충색보순대두이황동용액적순도무현저성변화,단농도균현저증가,분석인위분장용기적밀봉성시도치농도증가적원인。본연구결과위대두이황동단체용액표준용액산업화제공료기술지탱。
Six chromatographic grade soybean isoflavones solutions daidzin, glycitin, genistin, daidzein, glycitein, and genistein were prepared with a preparative high performance liquid chromatography (Shimadzu LC-20AP). The solution stabilities were monitored in changes of concentrations and purities during a one-month period under temperatures of –20℃, 4℃, 25℃, and 60℃, respectively, and during a six-month period under a temperature of 4℃. Variance analysis of the one-month period experimental results indicated that the concentrations of five soybean isoflavones solutions showed no obvious change under 4℃ and 25℃ except for genistin solution. No obvious changes in purities for all soybean isoflavones solutions under –20℃, 4°C, and 25℃ were observed. Temperatures of 4℃ and 25℃ were suggested for short-term storage of soybean isoflavones solutions. The t-test of the six-month period experimental results indicated that the concentrations of six chromatographic grade soybean isoflavones solutions increased significantly, but the purities of those did not change after 0, 1, 2, 3, 4, 6, 8, 12, 16, and 24 weeks preservation at 4℃. Leakage of methanol from containers of isoflavones solutions was considered to be the course of concentration increment. This research provided technical helps for the production of the soybean isoflavones standard solutions.