CAJ | 학술논문

菜籽饼是重要的饲料蛋白质来源,氨基酸组成与饲料营养品质有着密切关系,其中丝氨酸、胱氨酸和酪氨酸为多数动物的半必需氨基酸。本研究利用甘蓝型油菜双单倍体(DH)群体分别与双亲Tapidor和Ningyou 7回交构建的2套 BC1F1群体,采用新创建的双子叶作物种子品质性状遗传体系 QTL 定位软件和作图方法,对油菜籽丝氨酸、胱氨酸和酪氨酸含量进行了种子胚和母体植株2套遗传体系的QTL定位分析。结果表明,在A1、A4、A7、A8、A9、C2、C3和C9染色体上检测到5个丝氨酸含量QTL、2个胱氨酸含量QTL和5个酪氨酸含量QTL,分别解释59.34%、29.66%和59.26%的表型变异。其中5个QTL属于主效QTL,均能解释10%以上的表型变异。全部QTL均具极显著的胚和母体加性主效应,其中3个QTL具显著或极显著的环境互作效应。在A4染色体上发现1个QTL簇,该区域存在3个控制丝氨酸、胱氨酸和酪氨酸含量的QTL。一些重要QTL以及与之紧密连锁的分子标记在今后图位克隆和分子标记辅助选择育种中具有重要的利用价值。
채자병시중요적사료단백질래원,안기산조성여사료영양품질유착밀절관계,기중사안산、광안산화락안산위다수동물적반필수안기산。본연구이용감람형유채쌍단배체(DH)군체분별여쌍친Tapidor화Ningyou 7회교구건적2투 BC1F1군체,채용신창건적쌍자협작물충자품질성상유전체계 QTL 정위연건화작도방법,대유채자사안산、광안산화락안산함량진행료충자배화모체식주2투유전체계적QTL정위분석。결과표명,재A1、A4、A7、A8、A9、C2、C3화C9염색체상검측도5개사안산함량QTL、2개광안산함량QTL화5개락안산함량QTL,분별해석59.34%、29.66%화59.26%적표형변이。기중5개QTL속우주효QTL,균능해석10%이상적표형변이。전부QTL균구겁현저적배화모체가성주효응,기중3개QTL구현저혹겁현저적배경호작효응。재A4염색체상발현1개QTL족,해구역존재3개공제사안산、광안산화락안산함량적QTL。일사중요QTL이급여지긴밀련쇄적분자표기재금후도위극륭화분자표기보조선택육충중구유중요적이용개치。
Rapeseed meal is an important feed protein source, and the amino acid composition has a close relationship with the processing quality of feed, of which serine, cystine and tyrosine are the semi-essential amino acids for most animals. By using newly developed two-genetic-system QTL mapping software and method for analyzing seed quality traits of dicotyledonous plants, two backcross populations from a set of doubled haploid (DH) lines derived from an elite hybrid cross between Tapidor and Ningyou 7 were used to detect the QTLs simultaneously located in the amphidiploid embryo and maternal plant nuclear ge-nomes for the semi-essential amino acid contents of rapeseed across environments. The results showed that five QTLs for serine content, two QTLs for cystine content and five QTLs for tyrosine content were identified, which were subsequently mapped on chromosomes A1, A4, A7, A8, A9, C2, C3, or C9 and could respectively explain 59.34%, 29.66%, and 59.26% of phenotypic variation in total. Five QTLs were major effect QTLs which could explain more than 10% of phenotypic variation for each. All of these QTLs had both notable embryo and maternal additive main effects, among which three QTLs were also found to have sig-nificant QE interaction effects. One QTL cluster on chromosome A4 was discovered to contain three QTLs related to serine, cystine and tyrosine contents. Some important QTLs and the tightly linked markers will have an important application value in the later map-based cloning and marker-assisted selection.