CAJ | 학술논문

小麦新种质CH7124由八倍体小偃麦TAI8335与高感白粉病小麦品种晋麦33杂交后代衍生而来,在苗期对白粉病菌株E09、E20、E21、E23、E26、Bg1和Bg2表现免疫或高抗,抗病表现与TAI8335及其野生亲本中间偃麦草相似。基因组原位杂交未检测到CH7124含有外源染色体信号。利用CH7124与感病亲本SY95-71和绵阳11的杂交群体接种鉴定和遗传分析证实, CH7124成株期对E09的抗性由1对显性核基因控制,暂命名为PmCH7124。采用分离群体分组分析法(bulked segregant analysis, BSA)对SY95-71/CH7124的F6群体进行SSR标记扫描,发现抗性基因PmCH7124与5对SSR标记连锁,与两翼邻近标记Xgwm501和Xbarc101的遗传距离分别为1.7 cM和4.5 cM。利用中国春缺体–四体和双端体材料,将PmCH7124及其连锁标记定位在小麦2B染色体长臂上。通过分析2BL上其他抗白粉病基因的抗谱、抗性来源、物理图谱位置以及连锁标记在 PmCH7124作图群体中的多态性,认为 PmCH7124不同于2BL上已知的抗白粉病基因Pm6、Pm33、PmJM22、MlZec1、MlAB10和MlLX99。
소맥신충질CH7124유팔배체소언맥TAI8335여고감백분병소맥품충진맥33잡교후대연생이래,재묘기대백분병균주E09、E20、E21、E23、E26、Bg1화Bg2표현면역혹고항,항병표현여TAI8335급기야생친본중간언맥초상사。기인조원위잡교미검측도CH7124함유외원염색체신호。이용CH7124여감병친본SY95-71화면양11적잡교군체접충감정화유전분석증실, CH7124성주기대E09적항성유1대현성핵기인공제,잠명명위PmCH7124。채용분리군체분조분석법(bulked segregant analysis, BSA)대SY95-71/CH7124적F6군체진행SSR표기소묘,발현항성기인PmCH7124여5대SSR표기련쇄,여량익린근표기Xgwm501화Xbarc101적유전거리분별위1.7 cM화4.5 cM。이용중국춘결체–사체화쌍단체재료,장PmCH7124급기련쇄표기정위재소맥2B염색체장비상。통과분석2BL상기타항백분병기인적항보、항성래원、물리도보위치이급련쇄표기재 PmCH7124작도군체중적다태성,인위 PmCH7124불동우2BL상이지적항백분병기인Pm6、Pm33、PmJM22、MlZec1、MlAB10화MlLX99。
Wheat introgression line CH7124 derived from a cross between wheat–Thinopyrum intermedium line TAI8335 (resis-tant to powdery mildew) and common wheat variety “Jinmai 33” (susceptible to powdery mildew) exhibits immunity toBlumeria graminisf. sp.tritici (Bgt) pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2 at the seedling stage. TheBgt resistance in CH7124 is similar to that in TAI8335 and its wild parentTh. intermedium. However, noTh. intermedium chromatinhas been detected ac-cording to genome in situ hybridization (GISH) assay. In this study, we determined the single dominantBgt resistance gene in CH7124, tentatively designatedPmCH7124, using populations derived from SY95-71/CH7124 (F6) and CH7124/Mianyang 11 (F1 and F2). Five SSR markers (Xgwm47,Xgwm120,Xwmc332,Xgwm501, andXbarc101) were identified to be codominant with PmCH7124 according to bulked segregant analysis, and the closely flanking markers wereXgwm501 andXbacr101with genetic distances of 1.7 cM and 4.5 cM, respectively. The target resistance gene was chromosomally located on 2BL with Chinese Spring nulli-tetrasomicand ditelosomic lines. We primarily consider thatPmCH7124is a newBgt resistance gene because its resistance spectrum, origin, chromosomal location, and linked markers are different from those of the knownBgt resistance genes, such as Pm6,Pm33,PmJM22,MlZec1,MlAB10, andMlLX99.