CAJ | 학술논문

NAC 转录因子在植物应答非生物胁迫中起重要作用。利用生物信息学分析推测花生栽培种转录因子基因AhNAC4(登录号为 HM776131.1)属于抗旱相关转录因子基因,对比栽培品种山花11号 AhNAC4的2条 cDNA序列(ShrNAC4-a和ShrNAC4-b)及其相应的DNA序列(ShNAC4-a和ShNAC4-b)表明,AhNAC4全长为1244 bp,编码区长度为1050 bp,含有2个内含子,分别位于182~279 bp和547~642 bp处,编码蛋白包含349个氨基酸。从抗旱性不同的32个栽培品种分离得到4类AhNAC4,分别命名为AhNAC4-a1、AhNAC4-a2、AhNAC4-b1和AhNAC4-b2,缩写为a1、a2、b1和b2。a1和a2为等位基因,二者在717 bp处存在1个碱基差异,引起第174位氨基酸的改变,b1和b2为等位基因,二者存在14个SNP位点,其中717 bp和924 bp处碱基的差异引起第174位和第244位氨基酸的改变。供试品种中基因型为 a1a1b1b1、a1a1b2b2、a2a2b1b1、a2a2b2b2的品种数分别为10、5、15和2。从19个野生种中分离得到11类 NAC4的 DNA 序列(Aw1NAC4–Aw11NAC4), Aw1NAC4与栽培种 b1、b2的核苷酸序列同源性最高, Aw2NAC4与栽培种a1、a2核苷酸序列同源性最高。推测栽培种a1基因编码蛋白对花生抵御干旱起关键作用, a1和b1基因编码蛋白的功能与野生种更接近。
NAC 전록인자재식물응답비생물협박중기중요작용。이용생물신식학분석추측화생재배충전록인자기인AhNAC4(등록호위 HM776131.1)속우항한상관전록인자기인,대비재배품충산화11호 AhNAC4적2조 cDNA서렬(ShrNAC4-a화ShrNAC4-b)급기상응적DNA서렬(ShNAC4-a화ShNAC4-b)표명,AhNAC4전장위1244 bp,편마구장도위1050 bp,함유2개내함자,분별위우182~279 bp화547~642 bp처,편마단백포함349개안기산。종항한성불동적32개재배품충분리득도4류AhNAC4,분별명명위AhNAC4-a1、AhNAC4-a2、AhNAC4-b1화AhNAC4-b2,축사위a1、a2、b1화b2。a1화a2위등위기인,이자재717 bp처존재1개감기차이,인기제174위안기산적개변,b1화b2위등위기인,이자존재14개SNP위점,기중717 bp화924 bp처감기적차이인기제174위화제244위안기산적개변。공시품충중기인형위 a1a1b1b1、a1a1b2b2、a2a2b1b1、a2a2b2b2적품충수분별위10、5、15화2。종19개야생충중분리득도11류 NAC4적 DNA 서렬(Aw1NAC4–Aw11NAC4), Aw1NAC4여재배충 b1、b2적핵감산서렬동원성최고, Aw2NAC4여재배충a1、a2핵감산서렬동원성최고。추측재배충a1기인편마단백대화생저어간한기관건작용, a1화b1기인편마단백적공능여야생충경접근。
NAC transcription factors play an important role in response to abiotic stress of plant. In this paper, bioinformatic analysis indicated that transcription factor geneAhNAC4(accession number HM776131.1) can response to drought signal. The comparison of cDNAs (ShrNAC4-a andShrNAC4-b) and genomic DNAs (ShNAC4-a andShNAC4-b) from Shanhua 11 showed thatAhNAC4 has a full length of 1244 bp and an ORF of 1050 bp, containing two introns at 182–279 bp and 547–642 bp, and encoding 349 amino acids. Four kinds ofAhNAC4 genes, named asAhNAC4-a1, AhNAC4-a2, AhNAC4-b1, andAhNAC4-b2(ab-breviations:a1, a2, b1, andb2), were cloned from 32 cultivars with different drought resistances. Among them a1 anda2 were alleles, with only one locus different between the two genes, leading to the difference of corresponding amino acids at site 174;b1 andb2 were alleles, with 14 SNPs, of which two SNPs led to differences of the corresponding amino acids at sites 174 and 244. There were four genotypes ofAhNAC4 in the 32 cultivars, containing 10a1a1b1b1, 5a1a1b2b2, 15a2a2b1b1, and 2a2a2b2b2. Meanwhile, 11NAC4 genes (Aw1NAC4–Aw11NAC4) were isolated from 19 wild peanut accessions inArachis.Aw2NAC4 had the highest homology of nucleotide sequence with a1 ora2, andb1 orb2 had the highest homology of nucleotide sequence with Aw1NAC4. It is speculated that the protein encodinga1 plays a key role in responding drought stress, and the function of the pro-teins encodinga1 andb1 genes is closer with that of wild species.