CAJ | 학술논문

大豆胞囊线虫3号生理小种在我国已发现的8个小种中分布最为广泛，严重影响大豆生产。中品03-5373(ZP03-5373)是对3号小种免疫的优良抗源。本研究以中品03-5373为母本，与感病品种中黄13(ZH13)杂交建立包含254个家系的重组自交系群体，利用SSR、EST-SSR、InDel和SNP等506个分子标记对该分离群体进行基因型鉴定，构建全长为2651.90 cM的遗传图谱，标记间平均距离为5.24 cM。结合抗性鉴定数据，在中品03-5373中检测到3个控制大豆胞囊线虫3号生理小种的QTL区间，分别位于Gm07(SCN3-7)、Gm11(SCN3-11)和Gm18(SCN3-18)。其中SCN3-18可解释29.5%的抗性变异，为主效抗性位点； SCN3-7和SCN3-11分别控制6.2%和5.5%的抗性变异，为微效位点。SCN3-7与SCN3-18间存在显著的上位性互作。通过对中品03-5373祖先亲本2个QTL区间(SCN3-7和SCN3-11)侧翼标记的系谱追踪，进一步证明SCN3-7和SCN3-11与大豆胞囊线虫3号抗性相关。
대두포낭선충3호생리소충재아국이발현적8개소충중분포최위엄범，엄중영향대두생산。중품03-5373(ZP03-5373)시대3호소충면역적우량항원。본연구이중품03-5373위모본，여감병품충중황13(ZH13)잡교건립포함254개가계적중조자교계군체，이용SSR、EST-SSR、InDel화SNP등506개분자표기대해분리군체진행기인형감정，구건전장위2651.90 cM적유전도보，표기간평균거리위5.24 cM。결합항성감정수거，재중품03-5373중검측도3개공제대두포낭선충3호생리소충적QTL구간，분별위우Gm07(SCN3-7)、Gm11(SCN3-11)화Gm18(SCN3-18)。기중SCN3-18가해석29.5%적항성변이，위주효항성위점； SCN3-7화SCN3-11분별공제6.2%화5.5%적항성변이，위미효위점。SCN3-7여SCN3-18간존재현저적상위성호작。통과대중품03-5373조선친본2개QTL구간(SCN3-7화SCN3-11)측익표기적계보추종，진일보증명SCN3-7화SCN3-11여대두포낭선충3호항성상관。
Soybean cyst nematode (SCN) race 3, one of the eight races identified in China, is widely distributed and severely re-duced soybean yield. Zhongpin 03-5373 (ZP03-5373) is an elite line immune to SCN race 3. In this study, a recombinant inbred line (RIL) population was developed from a cross between ZP03-5373 and Zhonghuang 13 (ZH13). A genetic linkage map was constructed using a total of 506 molecular markers, including SSRs, EST-SSRs, InDel, and SNP. The total length of the genetic map was 2651.90 cM with an average marker spacing of 5.24 cM. Based on the phenotyping data, we detected three QTL inter-vals to dominate SCN3, includingSCN3-7 (Gm07),SCN3-11(Gm11), andSCN3-18(Gm11). Main effect QTL,SCN3-18 could explain 29.5% of resistant variation. Two minor effect QTLs,SCN3-7 andSCN3-11, explained 6.2% and 5.5% of resistant varia-tion, respectively. And it further showed there was significant epistatic interaction betweenSCN3-7 and SCN3-18 for resistance to SCN3. BothSCN3-7 andSCN3-11 were confirmed to be resistant to SCN3 by tracking flanking marker in the ancestors of ZP03-5373. These markers will be helpful for developing SCN resistant cultivars and cloning resistant genes by marker assisted selection.