CAJ | 학술논문

本课题组前期研究表明，过表达AhAREB1（花生AREB转录因子基因）植株矮小，叶片卷曲，抗旱能力增强，体内IAA含量升高，推测AhAREB1可能影响植株体内IAA分布。该文采用携带3个重复IAA化学诱导启动子元件的pDR5：GUS植株与AhAREB1过表达拟南芥植株的杂交纯合后代，通过GUS组织化学染色方法，探讨AhAREB1转录因子对植株体内IAA分布的影响。结果表明，过表达AhAREB1植株体内IAA增加，主要分布在幼苗的根尖和成苗的叶片边缘；在过表达植株IAA响应基因IAA29和IAA运输蛋白基因LAX3表达均显著下调，而IAA响应蛋白基因ARF2和ARF9显著上调。过表达AhAREB1植株体内IAA分布不均衡是引起表型变化的原因之一。
본과제조전기연구표명，과표체AhAREB1（화생AREB전록인자기인）식주왜소，협편권곡，항한능력증강，체내IAA함량승고，추측AhAREB1가능영향식주체내IAA분포。해문채용휴대3개중복IAA화학유도계동자원건적pDR5：GUS식주여AhAREB1과표체의남개식주적잡교순합후대，통과GUS조직화학염색방법，탐토AhAREB1전록인자대식주체내IAA분포적영향。결과표명，과표체AhAREB1식주체내IAA증가，주요분포재유묘적근첨화성묘적협편변연；재과표체식주IAA향응기인IAA29화IAA운수단백기인LAX3표체균현저하조，이IAA향응단백기인ARF2화ARF9현저상조。과표체AhAREB1식주체내IAA분포불균형시인기표형변화적원인지일。
Our previous study showed that Arabidopsis plants over-expressing AhAREB1 ( a peanut AREB transcrip-tion factor) displayed growth retardation, curly leaves, enhanced drought tolerance and increased IAA content.It is implied that auxin distribution may be affected by the expression of AhAREB1.In the present study, the homozy-gous transgenic Arabidopsis, generated by hybridizing the pDR5::GUS ( harboring GUS gene driven by a promoter with three repeat IAA-induced elements) plants with AhAREB1 over-expressing plants, was used to further investi-gate the effect of the expression of AhAREB1 transcription factor on auxin distribution through GUS histochemical staining .The results showed that the IAA content was increased in AhAREB1 over-expressing plants when compared with that in the controlled plants.The IAA mainly accumulated in the root tip of transgenic seedlings, instead on the edge of the leaves in mature plants.The expressions of IAA-responsive genes IAA29 and IAA-transport gene LAX3 in AhAREB1 over-expressing plants were significantly down-regulated, whereas The expressions of other two IAA-responsive genes ARF2 and ARF9 were significantly up-regulated.Take together, the unbalanced IAA distri-bution caused by the over-expression of AhAREB1 may be involved in the altered phenotype of transgenic plants.