CAJ | 학술논문

以色氨酸和4-氯-7-硝基苯并呋咱为原料，设计合成了一种碱性pH荧光探针，通过核磁、质谱表征了它的结构．光谱测试结果表明，当探针水溶液的pH值由7．94上升到12．24时，其紫外吸收光谱发生明显变化，480 nm处的吸收峰明显下降，同时在390 nm处出现一个新的吸收峰，探针水溶液的颜色由橙红色变为黄色；并且其荧光发射峰的强度逐渐下降，产生明显的猝灭现象．因此，该探针对碱性条件具有明显的光学响应现象．
이색안산화4-록-7-초기분병부찰위원료，설계합성료일충감성pH형광탐침，통과핵자、질보표정료타적결구．광보측시결과표명，당탐침수용액적pH치유7．94상승도12．24시，기자외흡수광보발생명현변화，480 nm처적흡수봉명현하강，동시재390 nm처출현일개신적흡수봉，탐침수용액적안색유등홍색변위황색；병차기형광발사봉적강도축점하강，산생명현적졸멸현상．인차，해탐침대감성조건구유명현적광학향응현상．
A new alkaline fluorescence probe, 7-nitro-benzoxadiazol-tryptophan, was synthesized.The experiment results indicated that when the pH value rose from 7.94 to 12.24, the UV-vis spectrum of the probe in aqueous so-lution changed obviously.The absorption peak of 480 nm quenched, meanwhile a new absorption peak appeared at 390 nm, and the color changed from orange-red to yellow.The intensity of fluorescent peak dropped gradually, which indicated that it was an evident quenching phenomenon.The probe had obvious optical response in alkaline condition.