CAJ | 학술논문

目的：比较两种核酸提取方法对实时荧光定量聚合酶链反应（FQ‐PCR）检测丙型肝炎病毒（HCV ） RNA病毒载量的影响。方法选择89例2012年10月至2013年6月在肝病中心住院的慢性丙型肝炎抗‐HCV阳性患者，分别用磁珠法和TRIZOL法提取血清标本中HCV RNA ，用FQ‐PCR技术检测 HCV RNA ，并对结果进行对比分析，统计学处理采用χ2检验和 t检验。结果89例抗 HCV阳性标本中以磁珠法提取核酸进行 HCV RNA定量检测的阳性率为73．0％（65／89），以 TRIZOL 法提取核酸进行 HCV RNA定量检测的阳性率为65．1％（58／89），两法提取方法检测阳性率比较有统计学意义（χ2＝3．76，P＜0．05）。两法所测浓度结果换算成对数值，经 t检验两者差异无统计学意义（t＝0．32，P＞0．05）。结论两种方法均可获得较高的回收率、有较高的敏感度和特异度，磁珠法提取 HCV RNA可用于HCV感染者的临床诊断和疗效观察。
목적：비교량충핵산제취방법대실시형광정량취합매련반응（FQ‐PCR）검측병형간염병독（HCV ） RNA병독재량적영향。방법선택89례2012년10월지2013년6월재간병중심주원적만성병형간염항‐HCV양성환자，분별용자주법화TRIZOL법제취혈청표본중HCV RNA ，용FQ‐PCR기술검측 HCV RNA ，병대결과진행대비분석，통계학처리채용χ2검험화 t검험。결과89례항 HCV양성표본중이자주법제취핵산진행 HCV RNA정량검측적양성솔위73．0％（65／89），이 TRIZOL 법제취핵산진행 HCV RNA정량검측적양성솔위65．1％（58／89），량법제취방법검측양성솔비교유통계학의의（χ2＝3．76，P＜0．05）。량법소측농도결과환산성대수치，경 t검험량자차이무통계학의의（t＝0．32，P＞0．05）。결론량충방법균가획득교고적회수솔、유교고적민감도화특이도，자주법제취 HCV RNA가용우HCV감염자적림상진단화료효관찰。
Objective To compare the influences of two kinds of nucleic acid extraction method for detecting hepatitis C virus(HCV) RNA viral load by the real‐time fluorescenct quantitative(FQ) PCR .Methods 89 inpatients with chronic hepatitis C (CHC) and anti‐HCV positive in the Therapy Center for Liver Diseases of our hospital from October 2012 to June 2013 were selected and HCVRNA was extracted from the serum specimens by using the mag‐netic beads and Trizol methods .FQ‐PCR was adopted for detecting serum HCV RNA .The detected results were per‐formed the comparative analysis .The statistic processing adopted the χ2 test and t test .Results The positive rate of serum HCV RNA extracted by the magnetic beads and Trizol methods were 73 .0% (65/89) and 65 .1% (58/89) re‐spectively ,and the difference between the two methods showed the statistical significance (χ2 =3 .76 ,P<0 .05) .By converting the concentration results detected by these two methods into the log values ,there was no statistically sig‐nificant difference between the two methods(t=0 .32 ,P>0 .05) .Conclusion The two kinds of method can obtain the higher recovery rate with higher sensitivity and higher specificity .The magnetic beads method for extracting HCV RNA could be used in the clinical diagnosis and therapeutic observation in the HCV infected persons .