CAJ | 학술논문

目的：探讨氯化钴（ CoCl2）诱导SH-SY5Y细胞缺氧损伤的作用及相关机制。方法用四甲基偶氮唑蓝比色法（ MTT）观察不同浓度CoCl2对SH-SY5Y细胞生长情况影响；ELISA法检测细胞内相关凋亡蛋白Bcl-2、Bax、Caspase-3及缺氧相关蛋白HIF-1α的表达情况。结果 CoCl2可以诱导SH-SY5Y细胞损伤，并呈现浓度和时间依赖性；CoCl2作用于SH-Y5Y细胞导致HIF-1α、Caspace-3和Bax的表达增多（P＜0．05），Bcl-2表达减少（P＜0．01）。结论 CoCl2诱导SH-SY5Y细胞缺氧损伤与上调HIF-1α表达、促进细胞凋亡有关。
목적：탐토록화고（ CoCl2）유도SH-SY5Y세포결양손상적작용급상관궤제。방법용사갑기우담서람비색법（ MTT）관찰불동농도CoCl2대SH-SY5Y세포생장정황영향；ELISA법검측세포내상관조망단백Bcl-2、Bax、Caspase-3급결양상관단백HIF-1α적표체정황。결과 CoCl2가이유도SH-SY5Y세포손상，병정현농도화시간의뢰성；CoCl2작용우SH-Y5Y세포도치HIF-1α、Caspace-3화Bax적표체증다（P＜0．05），Bcl-2표체감소（P＜0．01）。결론 CoCl2유도SH-SY5Y세포결양손상여상조HIF-1α표체、촉진세포조망유관。
Objective To investigate the hypoxia injury and mechanism of Cobalt chloride ( CoCl2 ) on SH-SY5Y cells.Methods Different doses of CoCl2 were used to treat SH-SY5Y cells,MTT assay was employed to examine the growth of the cells .ELISA was used to detect the expressions of Bax ,Bcl-2 ,Caspase-3 and HIF-1α.Results The in-hibitory effect of CoCl 2 was appeared in a time and dosage dependent manner .CoCl 2 could increase the expression of Caspase-3, Bax and HIF-1α(P<0.05),while reduce the expression of Bcl-2 (P<0.01).Conclusion CoCl2 could induce hypoxia on SH-SY5 Y cells by up-regulating HIF-1αand inducing apoptosis .