辽东学院学报(自然科学版)
遼東學院學報(自然科學版)
료동학원학보(자연과학판)
JOURNAL OF LIAODONG UNIVERSITY(NATURAL SCIENCES)
2015年
1期
23-26,47
,共5页
李娟%宋洁%姜路路%冯新雨%刘畅%单洪宇
李娟%宋潔%薑路路%馮新雨%劉暢%單洪宇
리연%송길%강로로%풍신우%류창%단홍우
草莓炭疽病%拮抗%枯草芽孢杆菌%筛选
草莓炭疽病%拮抗%枯草芽孢桿菌%篩選
초매탄저병%길항%고초아포간균%사선
strawberry anthracnose%antagonistic%Bacillus subtilis%screening
研究以草莓胶孢炭疽菌为指示菌,采用平板对峙法初筛、发酵液生长速率法复筛,自草莓根际土壤样品中获得一株对草莓炭疽病具有良好拮抗作用的细菌TJX-012。该菌株在平板上对草莓炭疽病菌的抑菌带宽度最大可达15 mm,发酵液抑菌实验表明该菌株抑菌率为67%。经形态学、生理生化特征测定及16S rDNA序列的系统发育分析,该菌株鉴定为枯草芽孢杆菌。
研究以草莓膠孢炭疽菌為指示菌,採用平闆對峙法初篩、髮酵液生長速率法複篩,自草莓根際土壤樣品中穫得一株對草莓炭疽病具有良好拮抗作用的細菌TJX-012。該菌株在平闆上對草莓炭疽病菌的抑菌帶寬度最大可達15 mm,髮酵液抑菌實驗錶明該菌株抑菌率為67%。經形態學、生理生化特徵測定及16S rDNA序列的繫統髮育分析,該菌株鑒定為枯草芽孢桿菌。
연구이초매효포탄저균위지시균,채용평판대치법초사、발효액생장속솔법복사,자초매근제토양양품중획득일주대초매탄저병구유량호길항작용적세균TJX-012。해균주재평판상대초매탄저병균적억균대관도최대가체15 mm,발효액억균실험표명해균주억균솔위67%。경형태학、생리생화특정측정급16S rDNA서렬적계통발육분석,해균주감정위고초아포간균。
From strawberry rhizosphere soil samples, Bacillus strain TJX-012 was obtained by plate confron-tation and fermentation liquid growth rate methods.TJX -012 has good antagonistic effects on Colletotrichum gloeosporioides.Its bacteriostatic band width was 15 mm on plate.Antimicrobial test showed that the strain inhibi-tory rate was as high as 67%.Based on the morphological, biochemical characteristics and 16s rDNA gene se-quence and phylogenetic analysis, the strain TJX-012 was identified as Bacillus subtilis.