CAJ | 학술논문

目的：观察重症急性胰腺炎大鼠肺损伤的病理生理变化并探讨其发生机制。方法选取2008年1月至2009年1月健康雄性SD（ Sprague-Dawley）大鼠240只，按照随机数字表随机均分为8组，每组30只，即重症急性胰腺炎（ SAP）不同时间点组（ SAP 1 h、3 h、6 h、9 h、12 h、18 h、24 h组）和对照组。比较各组血清淀粉酶、肺组织湿／干重比、肺髓过氧化物酶（ myeloperoxidase ，MPO）活性、肺通透性指数的变化。结果观察组大鼠肺损伤组织评分、肺组织湿干重比以及肺髓过氧化物酶（ MPO）水平随着时间的延续逐渐升高，各时间点组均明显高于对照组（ P ＜0^．05），观察组肺通透性指数随时间延续呈逐渐降低趋势，均低于对照组，差异均有统计学意义（ P ＜0．05）。肺组织MPO水平与肺损伤评分存在正相关（ r ＝0．946， P ＜0．01），肺湿干重比与肺损伤评分呈正相关（ r ＝0．879， P ＜0．01），肺通透性指数与肺损伤评分存在负相关（ r ＝－0．859， P ＜0．01）。结论重症急性胰腺炎大鼠肺损伤的损伤程度随SAP病程延长而逐渐增加，肺组织MPO水平持续升高。
목적：관찰중증급성이선염대서폐손상적병리생리변화병탐토기발생궤제。방법선취2008년1월지2009년1월건강웅성SD（ Sprague-Dawley）대서240지，안조수궤수자표수궤균분위8조，매조30지，즉중증급성이선염（ SAP）불동시간점조（ SAP 1 h、3 h、6 h、9 h、12 h、18 h、24 h조）화대조조。비교각조혈청정분매、폐조직습／간중비、폐수과양화물매（ myeloperoxidase ，MPO）활성、폐통투성지수적변화。결과관찰조대서폐손상조직평분、폐조직습간중비이급폐수과양화물매（ MPO）수평수착시간적연속축점승고，각시간점조균명현고우대조조（ P ＜0^．05），관찰조폐통투성지수수시간연속정축점강저추세，균저우대조조，차이균유통계학의의（ P ＜0．05）。폐조직MPO수평여폐손상평분존재정상관（ r ＝0．946， P ＜0．01），폐습간중비여폐손상평분정정상관（ r ＝0．879， P ＜0．01），폐통투성지수여폐손상평분존재부상관（ r ＝－0．859， P ＜0．01）。결론중증급성이선염대서폐손상적손상정도수SAP병정연장이축점증가，폐조직MPO수평지속승고。
Objective To observe the pathophysiological change of acute lung injury in rats with severe acute pancreatitis(SAP),and to explore its pathogenesis mechanism .Methods The 240 healthy male SD(Sprague-Dawley) rats from January 2008 to 2009 January were enrolled in the study , these rats were randomly divided into 8 groups according to random digits table,7 observation groups and control group ,the observation groups included 7 different time points of SAP ,ie. 1h group,3h group,6h group,9h group,12h group,18h group,24h group and control group ,with 30 rats in each group.The changes of serum amylase , the ratio of lung tissue wet/dry weight , lung myeloperoxidase ( MPO ) activity and the lungs permeability index were detected and compared among the 8 groups.Results The lung injury score,the ratio of lung tissue wet/dry weight and lung MPO levels in observation groups were gradually increased ,as compared with those in control group , moreover,which in 7 time points were significantly higher than those in control group ( P <0.05).The lung permeability index in observation groups was gradually decreased ,as compared with that in control group ( P <0.05).The MPO levels in lung tissue were positively correlated to lung injury score ( r =0.946, P <0.01),and the ratio of lung tissue wet/dry weight was positively correlated to lung injury score ( r =0.879, P <0.01),however,lungs permeability index was negatively correlated to lung injury score( r =0.859, P <00.1 ).Conclu sion The lung injury degree of rats with SAP is gradually increased with prolongation of course of disease ,moreover ,the MPO levels in lung tissue are continually increased .