河北医药
河北醫藥
하북의약
HEBEI MEDICAL JOURNAL
2015年
3期
331-333
,共3页
巨噬细胞移动抑制因子%MIF-173G/C多态性%银屑病
巨噬細胞移動抑製因子%MIF-173G/C多態性%銀屑病
거서세포이동억제인자%MIF-173G/C다태성%은설병
macrophage migration inhibition factor%MIF-173 G/C polymorphism%psoriasis
目的：探讨恩施地区土家族银屑病患者巨噬细胞移动抑制因子（ migration inhibitory factor ，MIF）表达水平，分析M IF173 G／C多态性与银屑病发病关联性。方法对2013年2月至2014年2月恩施中心医院收治的245例银屑病土家族患者及260名土家族健康对照（对照组），应用 SNaPshot SNP 分型技术确定MIF-173 G／C等位基因型， ELISA法检测血清中MIF水平。比较病例组和对照组MIF 表达水平以及MIF-173G／C 基因型和等位基因频率的差异。结果银屑病组MIF水平明显高于对照组（ P ＜0？．01）； MIF基因173 G ／C基因型和等位基因频率在2组间分布差异有统计学意义（ P ＜0．05），银屑病组CC 基因型和C等位基因频率均显著高于对照组（ P ＜0．01）；相对于GG基因型，暴露于CG 、CC基因型的相对危险度分别为1．46（95％CI：1．01～2．10， P ＜0．05）和5．19（95％CI：1．69～15．89， P ＜0．01）；MIF173G／C多态性3种基因型GG、GC、CC的银屑病患者的临床特征比较，显示家族史、寻常型银屑病差异有统计学意义（ P ＜0．05）。结论 MIF-173G／C可能是恩施地区土家族银屑病患者发病的遗传危险因素之一，其机制是该多态性对影响MIF表达水平。
目的：探討恩施地區土傢族銀屑病患者巨噬細胞移動抑製因子（ migration inhibitory factor ，MIF）錶達水平，分析M IF173 G／C多態性與銀屑病髮病關聯性。方法對2013年2月至2014年2月恩施中心醫院收治的245例銀屑病土傢族患者及260名土傢族健康對照（對照組），應用 SNaPshot SNP 分型技術確定MIF-173 G／C等位基因型， ELISA法檢測血清中MIF水平。比較病例組和對照組MIF 錶達水平以及MIF-173G／C 基因型和等位基因頻率的差異。結果銀屑病組MIF水平明顯高于對照組（ P ＜0？．01）； MIF基因173 G ／C基因型和等位基因頻率在2組間分佈差異有統計學意義（ P ＜0．05），銀屑病組CC 基因型和C等位基因頻率均顯著高于對照組（ P ＜0．01）；相對于GG基因型，暴露于CG 、CC基因型的相對危險度分彆為1．46（95％CI：1．01～2．10， P ＜0．05）和5．19（95％CI：1．69～15．89， P ＜0．01）；MIF173G／C多態性3種基因型GG、GC、CC的銀屑病患者的臨床特徵比較，顯示傢族史、尋常型銀屑病差異有統計學意義（ P ＜0．05）。結論 MIF-173G／C可能是恩施地區土傢族銀屑病患者髮病的遺傳危險因素之一，其機製是該多態性對影響MIF錶達水平。
목적：탐토은시지구토가족은설병환자거서세포이동억제인자（ migration inhibitory factor ，MIF）표체수평，분석M IF173 G／C다태성여은설병발병관련성。방법대2013년2월지2014년2월은시중심의원수치적245례은설병토가족환자급260명토가족건강대조（대조조），응용 SNaPshot SNP 분형기술학정MIF-173 G／C등위기인형， ELISA법검측혈청중MIF수평。비교병례조화대조조MIF 표체수평이급MIF-173G／C 기인형화등위기인빈솔적차이。결과은설병조MIF수평명현고우대조조（ P ＜0？．01）； MIF기인173 G ／C기인형화등위기인빈솔재2조간분포차이유통계학의의（ P ＜0．05），은설병조CC 기인형화C등위기인빈솔균현저고우대조조（ P ＜0．01）；상대우GG기인형，폭로우CG 、CC기인형적상대위험도분별위1．46（95％CI：1．01～2．10， P ＜0．05）화5．19（95％CI：1．69～15．89， P ＜0．01）；MIF173G／C다태성3충기인형GG、GC、CC적은설병환자적림상특정비교，현시가족사、심상형은설병차이유통계학의의（ P ＜0．05）。결론 MIF-173G／C가능시은시지구토가족은설병환자발병적유전위험인소지일，기궤제시해다태성대영향MIF표체수평。
Objective To investigate the levels of macrophage migration inhibitory factor ( MIF) in serum of patients with psoriasis in Tujia population of Enshi District , and to explore the correlation between the pathogenesis of psoriasis and 173 G/C polymorphism .Methods A total of 245 patients with psoriasis ( case group ) who were admitted to our hospital from September 2009 to September 2010 and 260 healthy volunteers of Tujia population in the same period were enrolled in the study.The serum levels of MIF were detected by ELISA , and MIF-173 G/C was genetyped by SNaPshot SNP technique . Finally,MIF-173 G/C genotype and serum levels of MIF were analyzed and compared between the two groups .Results The serum levels of MIF in case group were significantly higher than those in control group [(804.21 ±69.78)pg/ml vs (369.46 ±60.46)pg/ml, P <0.01].There were significant differences in the 173G/C genotype of MIF gene and allele frequency between two groups ( P <0.05).The CC genotype and C allele frequency in case group were significantly higher than those in control group ( P <0.01).As compared with GG genotype,the relative risk exposed to CG,CC genetype was 1.46 (95%CI:1.01~2.10, P <0.05),5.19 ( 95%CI:1.69~15.89, P <0.01),respectively.The clinical characteristics were compared in the patients with three different kinds of genotype (GG,GC,CC) of MIF173G/C polymorphism,which showed that there was a significant difference in family history ,psoriasis vulgaris ( P <0 .05 ) .Conclusion MIF-173 G/C polymorphism may be one of genetic susceptibility factors in pathogenesis of psoriasis in Tujia population of Enshi District , and its potential mechanism is that the polymorphism may affect the expression levels of MIF .
