湘南学院学报（医学版）
湘南學院學報（醫學版）
상남학원학보（의학판）
JOURNAL OF XIANGNAN UNIVERSITY(MEDICAL SCIENCES)
2014年
4期
5-7
,共3页
5-氮杂-2ˊ-脱氧胞苷%肝癌细胞%RASSF1A基因%启动子甲基化
5-氮雜-2ˊ-脫氧胞苷%肝癌細胞%RASSF1A基因%啟動子甲基化
5-담잡-2ˊ-탈양포감%간암세포%RASSF1A기인%계동자갑기화
5-Aza-CdR%Hepatoma cells%RASSFIA gene%Promoter methylation
目的：探讨5-氮杂-2ˊ-脱氧胞苷(5-Aza-CdR)对人肝癌细胞RASSF1A基因启动子甲基化以及mRNA表达的影响。方法体外培养人肝癌细胞株SMMC-7721,分别用0.5、5.0、30.0、50.0μmol/L的5-Aza-CdR与之孵育72 h,甲基化PCR检测处理前后启动子甲基化水平,RT-PCR检测RASSF1A mRNA表达。结果随着药物浓度的增加,甲基化RASSF1A含量逐渐降低,而未甲基化产物逐渐增高；5-Aza-CdR处理后,RASSF1A mRNA的含量随之增高。结论5-Aza-CdR可逆转SMMC-7721细胞株细胞中基因甲基化修饰状态。
目的：探討5-氮雜-2ˊ-脫氧胞苷(5-Aza-CdR)對人肝癌細胞RASSF1A基因啟動子甲基化以及mRNA錶達的影響。方法體外培養人肝癌細胞株SMMC-7721,分彆用0.5、5.0、30.0、50.0μmol/L的5-Aza-CdR與之孵育72 h,甲基化PCR檢測處理前後啟動子甲基化水平,RT-PCR檢測RASSF1A mRNA錶達。結果隨著藥物濃度的增加,甲基化RASSF1A含量逐漸降低,而未甲基化產物逐漸增高；5-Aza-CdR處理後,RASSF1A mRNA的含量隨之增高。結論5-Aza-CdR可逆轉SMMC-7721細胞株細胞中基因甲基化脩飾狀態。
목적：탐토5-담잡-2ˊ-탈양포감(5-Aza-CdR)대인간암세포RASSF1A기인계동자갑기화이급mRNA표체적영향。방법체외배양인간암세포주SMMC-7721,분별용0.5、5.0、30.0、50.0μmol/L적5-Aza-CdR여지부육72 h,갑기화PCR검측처리전후계동자갑기화수평,RT-PCR검측RASSF1A mRNA표체。결과수착약물농도적증가,갑기화RASSF1A함량축점강저,이미갑기화산물축점증고；5-Aza-CdR처리후,RASSF1A mRNA적함량수지증고。결론5-Aza-CdR가역전SMMC-7721세포주세포중기인갑기화수식상태。
Objective To explore the influence of 5-Aza-CdR on the methylation of RASSFIA gene pro_moter and the expression of mRNA. Methods Human hepatoma cells SMMC-7721 were cultured in vitro, and were treated by 0. 5, 5. 0, 30. 0, 50. 0 μmol/L of 5-Aza-CdR for 72h. With the methylation PCR de_tected the methylation level before and after treatment. And the methylation of RASSF1A were detected by PCR. The expression mRNA was detected by RT-PCR. Results The content of RASSFIA methylation de_creased gradually with the increase of the drug concentration, while the non-methylation product increased. In addition, the content of RASSF1A mRNA was also increased after the treatment with 5-Aza-CdR. Con―clusion 5-Aza-CdR could reverse the methylation modified state in SMMC-7721 cells.
