浙江创伤外科
浙江創傷外科
절강창상외과
ZHEJIANG JOURNAL OF TRAUMATIC SURGERY
2014年
6期
873-876
,共4页
雷伟%宋振全%梁国标%李宾%赵旭%李晓明%柳云恩%吕伟%刘宏波%蒋为
雷偉%宋振全%樑國標%李賓%趙旭%李曉明%柳雲恩%呂偉%劉宏波%蔣為
뢰위%송진전%량국표%리빈%조욱%리효명%류운은%려위%류굉파%장위
5-HT1A受体激动剂%丁螺环酮%创伤性脑损伤%细胞凋亡%Bax%Bcl-2%Caspase-3
5-HT1A受體激動劑%丁螺環酮%創傷性腦損傷%細胞凋亡%Bax%Bcl-2%Caspase-3
5-HT1A수체격동제%정라배동%창상성뇌손상%세포조망%Bax%Bcl-2%Caspase-3
5-HT1A receptor agonist%Buspirone%Traumatic brain injury%Apoptosis%Bax%Bcl-2%Caspase-3
目的：探讨5-HT1A受体激动剂丁螺环酮对大鼠局灶性脑挫裂伤（Controlled cortical impact injury，CCII）损伤周围区域神经凋亡的影响。方法119只大鼠随机分为5组，A：空白对照组；B：空白对照+丁螺环酮组；C：假手术组；D：局灶性脑挫裂伤组+NS对照组；E：局灶性挫裂伤+丁螺环酮治疗组。参照改良Feeney's自由落体硬膜外撞击法制作局灶性脑挫裂伤模型，伤后1小时予以腹腔注射丁螺环酮（0.3mg/Kg)或等体积生理盐水（Normal saline ，NS)，在规定时间点，灌注取脑、石蜡包埋、切片，HE染色观察损伤周围区域皮质病理表现；免疫组化检测损伤灶周围皮层脑组织Bax、Bcl-2及Caspase-3蛋白的表达；TUNEL法检测损伤皮层凋亡细胞。结果 A、B、C组各指标阳性表现很弱，各指标没有明显的差异（P>0.05）；D、E组各时间点各指标与A、B、C组比较有明显差异（P<0.01）；D、E组之间各时间点各指标比较也有明显的统计学意义（P<0.05或P<0.01）。结论丁螺环酮对大鼠局灶性脑损伤后损伤灶周围皮层的神经细胞凋亡具有抑制作用。
目的：探討5-HT1A受體激動劑丁螺環酮對大鼠跼竈性腦挫裂傷（Controlled cortical impact injury，CCII）損傷週圍區域神經凋亡的影響。方法119隻大鼠隨機分為5組，A：空白對照組；B：空白對照+丁螺環酮組；C：假手術組；D：跼竈性腦挫裂傷組+NS對照組；E：跼竈性挫裂傷+丁螺環酮治療組。參照改良Feeney's自由落體硬膜外撞擊法製作跼竈性腦挫裂傷模型，傷後1小時予以腹腔註射丁螺環酮（0.3mg/Kg)或等體積生理鹽水（Normal saline ，NS)，在規定時間點，灌註取腦、石蠟包埋、切片，HE染色觀察損傷週圍區域皮質病理錶現；免疫組化檢測損傷竈週圍皮層腦組織Bax、Bcl-2及Caspase-3蛋白的錶達；TUNEL法檢測損傷皮層凋亡細胞。結果 A、B、C組各指標暘性錶現很弱，各指標沒有明顯的差異（P>0.05）；D、E組各時間點各指標與A、B、C組比較有明顯差異（P<0.01）；D、E組之間各時間點各指標比較也有明顯的統計學意義（P<0.05或P<0.01）。結論丁螺環酮對大鼠跼竈性腦損傷後損傷竈週圍皮層的神經細胞凋亡具有抑製作用。
목적：탐토5-HT1A수체격동제정라배동대대서국조성뇌좌렬상（Controlled cortical impact injury，CCII）손상주위구역신경조망적영향。방법119지대서수궤분위5조，A：공백대조조；B：공백대조+정라배동조；C：가수술조；D：국조성뇌좌렬상조+NS대조조；E：국조성좌렬상+정라배동치료조。삼조개량Feeney's자유락체경막외당격법제작국조성뇌좌렬상모형，상후1소시여이복강주사정라배동（0.3mg/Kg)혹등체적생리염수（Normal saline ，NS)，재규정시간점，관주취뇌、석사포매、절편，HE염색관찰손상주위구역피질병리표현；면역조화검측손상조주위피층뇌조직Bax、Bcl-2급Caspase-3단백적표체；TUNEL법검측손상피층조망세포。결과 A、B、C조각지표양성표현흔약，각지표몰유명현적차이（P>0.05）；D、E조각시간점각지표여A、B、C조비교유명현차이（P<0.01）；D、E조지간각시간점각지표비교야유명현적통계학의의（P<0.05혹P<0.01）。결론정라배동대대서국조성뇌손상후손상조주위피층적신경세포조망구유억제작용。
Objective To explore the neural cells apoptosis effect of 5-HT1A receptor agonist Buspirone on controlled cortical impact injury (CCII) rat. Methods 119 adult male Wister rats weighing 250 ±25g were randomly divided into 5 groups. A: Normal control group, n=7; B: Normal control+Buspirone group, n=7;C:Sham-operated group, n=35;D:CCII+Normal Saline;E:CCII+Buspirone. According to the time of take the brain, D or E group were divided into 6h, 12h, 24h, 72h, 168h, five subgroups, each 7 rats. 1 h after injury, Buspirone (0.3mg/kg) or equal volume NS were intraperitoneal injected. At the time point, the brain was taken and the pathological change of tissue around the injuried area was observed by H-E staining. The expression of Bax, BCL-2 and Caspase-3 protein were detected by immunohistochemical method. cortical cell apoptosis was detected by TUNEL method. Results There was no differences between A, B and C group (P>0.05). There was significant difference in each index respectively of D, E groups compared with those in A, B or C groups (P<0.01). the comparison between D and E of each index also had obvious statistical signifi-cance (P<0.05 or P<0.01). Conclusion Acute treatment with the 5-HT1A receptor agonist Buspirone had an effect of inhibition on neural cells apoptosis in controlled cortical impact injury rats.
