皖南医学院学报
皖南醫學院學報
환남의학원학보
ACTA ACADEMIAE MEDICINAE WANNAN
2014年
6期
474-477
,共4页
茶多糖%黑色素瘤%免疫%抑瘤率
茶多糖%黑色素瘤%免疫%抑瘤率
다다당%흑색소류%면역%억류솔
tea polysaccharides%melanoma%immunization%tumor inhibition rate
目的：观察茶多糖（TPS）对实验小鼠黑色素B16F10细胞瘤治疗效应，探讨茶多糖抑制黑色素细胞瘤的免疫学机制。方法：将C57BL／6随机分组，建立小鼠黑色素细胞瘤模型，通过持续灌胃服用茶多糖28 d，观察并记录荷瘤小鼠一般情况、肿瘤体积变化，最终检测实验小鼠NK细胞活性以及IFN-γ和IL-4分泌水平。结果：①TPS治疗组延缓了小鼠体质量改变，差异均有统计学意义（P＜0.05），以中剂量治疗组尤为明显（P＜0.01）；②TPS治疗组小鼠肿瘤直径与对照组比较差异均有统计学意义（P＜0.05），以中剂量治疗组差异最为显著（P＜0.01），中剂量抑瘤率最好；③TPS治疗组的NK细胞活性在效靶比为20∶1时，与对照组比较，差异均有统计学意义（P＜0.05），中剂量治疗组的NK活性达39.06％，差异有显著统计学意义（P＜0.01）；④TPS治疗组IFN-γ分泌水平均显示升高，而IL-4分泌水平则呈现下降，与对照组比较差异均有统计学意义（ P＜0.05），以中剂量治疗组变化尤为显著（P＜0.01）。结论：在本实验条件下，TPS对荷瘤小鼠显示出较为有效的治疗效应，以中剂量治疗组（400 mg／kg）效果最好，其机制可能与增强小鼠NK细胞活性、增加IFN-γ以及降低IL-4分泌水平等免疫调节有关。
目的：觀察茶多糖（TPS）對實驗小鼠黑色素B16F10細胞瘤治療效應，探討茶多糖抑製黑色素細胞瘤的免疫學機製。方法：將C57BL／6隨機分組，建立小鼠黑色素細胞瘤模型，通過持續灌胃服用茶多糖28 d，觀察併記錄荷瘤小鼠一般情況、腫瘤體積變化，最終檢測實驗小鼠NK細胞活性以及IFN-γ和IL-4分泌水平。結果：①TPS治療組延緩瞭小鼠體質量改變，差異均有統計學意義（P＜0.05），以中劑量治療組尤為明顯（P＜0.01）；②TPS治療組小鼠腫瘤直徑與對照組比較差異均有統計學意義（P＜0.05），以中劑量治療組差異最為顯著（P＜0.01），中劑量抑瘤率最好；③TPS治療組的NK細胞活性在效靶比為20∶1時，與對照組比較，差異均有統計學意義（P＜0.05），中劑量治療組的NK活性達39.06％，差異有顯著統計學意義（P＜0.01）；④TPS治療組IFN-γ分泌水平均顯示升高，而IL-4分泌水平則呈現下降，與對照組比較差異均有統計學意義（ P＜0.05），以中劑量治療組變化尤為顯著（P＜0.01）。結論：在本實驗條件下，TPS對荷瘤小鼠顯示齣較為有效的治療效應，以中劑量治療組（400 mg／kg）效果最好，其機製可能與增彊小鼠NK細胞活性、增加IFN-γ以及降低IL-4分泌水平等免疫調節有關。
목적：관찰다다당（TPS）대실험소서흑색소B16F10세포류치료효응，탐토다다당억제흑색소세포류적면역학궤제。방법：장C57BL／6수궤분조，건립소서흑색소세포류모형，통과지속관위복용다다당28 d，관찰병기록하류소서일반정황、종류체적변화，최종검측실험소서NK세포활성이급IFN-γ화IL-4분비수평。결과：①TPS치료조연완료소서체질량개변，차이균유통계학의의（P＜0.05），이중제량치료조우위명현（P＜0.01）；②TPS치료조소서종류직경여대조조비교차이균유통계학의의（P＜0.05），이중제량치료조차이최위현저（P＜0.01），중제량억류솔최호；③TPS치료조적NK세포활성재효파비위20∶1시，여대조조비교，차이균유통계학의의（P＜0.05），중제량치료조적NK활성체39.06％，차이유현저통계학의의（P＜0.01）；④TPS치료조IFN-γ분비수평균현시승고，이IL-4분비수평칙정현하강，여대조조비교차이균유통계학의의（ P＜0.05），이중제량치료조변화우위현저（P＜0.01）。결론：재본실험조건하，TPS대하류소서현시출교위유효적치료효응，이중제량치료조（400 mg／kg）효과최호，기궤제가능여증강소서NK세포활성、증가IFN-γ이급강저IL-4분비수평등면역조절유관。
Objective:To observe the therapeutic effects of tea polysaccharide (TPS) on B16F10 melanoma cells in experimental mice,and investigate the immunological mechanisms TPS in inhibiting such tumor cells .Methods:Female C57BL/6 mice,aged 6 weeks and weighed 12 to 14g ,were randomized into four groups(n=8 for each).Melanoma models were developed,and administered with intragastric TPS for consecutive 28 days.The tumor-bearing mice were observed and recorded regarding the changes in general condition and tumor volume .Finally,the sera were obtained from the experimental mice to determine the NK cell activity as well as IFN-γand IL-4 levels.Results:① TPS was capable of delaying weight loss of mice,and the effect was obvious in animals treated with medium dose(P<0.05,or P<0.01);②The tumor diameter was different,especially significant in medium dose treatment group, as compared with the controls(P<0.05,or P<0.01),especially that of the middle dose group;③NK cell activity was significantly different in TPS treat-ment group from that of the controls when the optimal effector-target ratio was set at 20∶1(P<0.05),and the activity reached 39.06% in mice adminis-tered with medium dosage(P<0.01);④Although elevated IFN-γlevel and decreased IL-4 level were observed in animals treated with TPS,yet the chan-ges were significant in those managed with medium dose(P <0.05,or P<0.01).Conclusion:TPS may facilitate therapeutic effects on tumor-bearing mice under our established experimental conditions ,and the effects are optimal in dose of 400 mg/kg.The potential mechanisms may be involved in boosted NK cell activity,increased IFN-γsecretion,but decreased IL-4 level through immune regulation.
