CAJ | 학술논문

建立一种微波消解辅助浊点萃取-同步荧光法测定海洋沉积物中的3,4-苯并(a)芘的新方法．通过加入TritonX-114,采用浊点萃取法对微波消解后的海洋污泥样品中痕量苯并(a)芘进行浓缩萃取,并结合灵敏的同步荧光法测定其含量．探讨影响苯并(a)芘萃取效果的因素,研究发现：最佳波长差为23 nm 时,苯并(a)芘乙醇溶液在384 nm处有一明显的同步荧光峰,BaP的浓度在0．01~0．2 ppm 范围内与荧光峰强度呈良好的线性关系,线性方程为I＝1．35＋701．77C(ppm),相关系数为0．9998,方法检出限为2．16 ppb．对照传统的甲苯溶剂超声波萃取法的实验结果,该方法具有线性范围宽、检出限低10倍、操作安全无毒的优点．
건립일충미파소해보조탁점췌취-동보형광법측정해양침적물중적3,4-분병(a)비적신방법．통과가입TritonX-114,채용탁점췌취법대미파소해후적해양오니양품중흔량분병(a)비진행농축췌취,병결합령민적동보형광법측정기함량．탐토영향분병(a)비췌취효과적인소,연구발현：최가파장차위23 nm 시,분병(a)비을순용액재384 nm처유일명현적동보형광봉,BaP적농도재0．01~0．2 ppm 범위내여형광봉강도정량호적선성관계,선성방정위I＝1．35＋701．77C(ppm),상관계수위0．9998,방법검출한위2．16 ppb．대조전통적갑분용제초성파췌취법적실험결과,해방법구유선성범위관、검출한저10배、조작안전무독적우점．
A new method synchronous fluorescence for the determination of trace 3 ,4-Benzo (a)pyrene (BaP)in Ocean Sediments was proposed by cloud point extracting which assisted with microwave di-gestion.The cloud point extraction method is adopted for marine sludge sample by using 5%(V/V) tritonX-114 solution with assistance of ultrasound,and the trace benzo(a)pyrene was extracted into nonaqueous phase,combined with the sensitivity of synchronous fluorescence method to measure its content in order to explore influence of benzo(a)pyrene extraction effect factors.The study found that fluorescence spectra BaP alcoholic solution has a strong and sharp synchronous fluorescence peak at 384 nm with optimal △λ=23 nm.The relative fluorescence peak intensity and the concentration of BaP show a good linear relationship in range of 0.01~0.2 ppm,linear equation I=1.35+701.77C(ppm) with the correlation coefficient r=0.999 8,the detection of the melamine limits to 2.16 ppb(3 S/K). Contrast to the experimental results of traditional liquid-liquid extraction method with toluene sol-vent,this method has the advantages of high sensitivity,low detection limit and safe non-toxic operat-ing.