泉州师范学院学报
泉州師範學院學報
천주사범학원학보
JOURNAL OF QUANZHOU NORMAL COLLGEG
2014年
6期
30-33
,共4页
谢晓兰%吴优%高平章%彭世旺%蒋乐杰
謝曉蘭%吳優%高平章%彭世旺%蔣樂傑
사효란%오우%고평장%팽세왕%장악걸
凡纳滨对虾%NAGase%辛基酚%酶活力
凡納濱對蝦%NAGase%辛基酚%酶活力
범납빈대하%NAGase%신기분%매활력
Litopenaeus vannamei%N-acetylglucosiminidase%Octyl-phenol%Enzyme activity
以凡纳滨对虾为研究对象,让其暴露在含0,5,15,45,135μg/L辛基酚(OP)的养殖水体中,暴露24,48,72,96,120 h后取样,分析测定凡纳滨对虾壳膜与内脏 NAGase 比活力的变化．结果表明,水体 OP 对对虾壳膜 NAGase比活力基本起激活作用．暴露72 h内,壳膜 NAGase比活力无显著性变化；暴露96 h时,5,15,135μg/L OP均对虾壳膜 NAGase 比活力起极显著激活作用(P＜0．01),45μg/L OP 对虾壳膜 NAGase比活力起显著激活(P＜0．05)；暴露120 h时,15,135μg/L OP 对虾壳膜 NAGase 比活力仍表现为显著性激活作用(P＜0．05),但5,45μg/L OP 对壳膜 NAGase 的比活力影响无显著性．而水体 OP 对虾内脏 NAGase比活力基本起抑制作用．在15μg/L OP体系下暴露24 h,内脏NAGase极显著下降(P＜0．01),其他 OP浓度下无显著性变化；不同浓度下暴露48 h,对虾内脏 NAGase比活力均出现极显著下降(P＜0．01)；5μg/L OP体系下暴露72 h,对虾内脏 NAGase比活力无显著性下降,其他 OP 浓度下的对虾内脏 NAGase 比活力均出现极显著下降(P＜0．01)；15,135μg/L OP 体系下暴露96 h,内脏 NAGase 比活力也分别出现极显著(P＜0．01)及显著性(P＜0．05)下降．可见,OP暴露对对虾生长发育会产生影响．
以凡納濱對蝦為研究對象,讓其暴露在含0,5,15,45,135μg/L辛基酚(OP)的養殖水體中,暴露24,48,72,96,120 h後取樣,分析測定凡納濱對蝦殼膜與內髒 NAGase 比活力的變化．結果錶明,水體 OP 對對蝦殼膜 NAGase比活力基本起激活作用．暴露72 h內,殼膜 NAGase比活力無顯著性變化；暴露96 h時,5,15,135μg/L OP均對蝦殼膜 NAGase 比活力起極顯著激活作用(P＜0．01),45μg/L OP 對蝦殼膜 NAGase比活力起顯著激活(P＜0．05)；暴露120 h時,15,135μg/L OP 對蝦殼膜 NAGase 比活力仍錶現為顯著性激活作用(P＜0．05),但5,45μg/L OP 對殼膜 NAGase 的比活力影響無顯著性．而水體 OP 對蝦內髒 NAGase比活力基本起抑製作用．在15μg/L OP體繫下暴露24 h,內髒NAGase極顯著下降(P＜0．01),其他 OP濃度下無顯著性變化；不同濃度下暴露48 h,對蝦內髒 NAGase比活力均齣現極顯著下降(P＜0．01)；5μg/L OP體繫下暴露72 h,對蝦內髒 NAGase比活力無顯著性下降,其他 OP 濃度下的對蝦內髒 NAGase 比活力均齣現極顯著下降(P＜0．01)；15,135μg/L OP 體繫下暴露96 h,內髒 NAGase 比活力也分彆齣現極顯著(P＜0．01)及顯著性(P＜0．05)下降．可見,OP暴露對對蝦生長髮育會產生影響．
이범납빈대하위연구대상,양기폭로재함0,5,15,45,135μg/L신기분(OP)적양식수체중,폭로24,48,72,96,120 h후취양,분석측정범납빈대하각막여내장 NAGase 비활력적변화．결과표명,수체 OP 대대하각막 NAGase비활력기본기격활작용．폭로72 h내,각막 NAGase비활력무현저성변화；폭로96 h시,5,15,135μg/L OP균대하각막 NAGase 비활력기겁현저격활작용(P＜0．01),45μg/L OP 대하각막 NAGase비활력기현저격활(P＜0．05)；폭로120 h시,15,135μg/L OP 대하각막 NAGase 비활력잉표현위현저성격활작용(P＜0．05),단5,45μg/L OP 대각막 NAGase 적비활력영향무현저성．이수체 OP 대하내장 NAGase비활력기본기억제작용．재15μg/L OP체계하폭로24 h,내장NAGase겁현저하강(P＜0．01),기타 OP농도하무현저성변화；불동농도하폭로48 h,대하내장 NAGase비활력균출현겁현저하강(P＜0．01)；5μg/L OP체계하폭로72 h,대하내장 NAGase비활력무현저성하강,기타 OP 농도하적대하내장 NAGase 비활력균출현겁현저하강(P＜0．01)；15,135μg/L OP 체계하폭로96 h,내장 NAGase 비활력야분별출현겁현저(P＜0．01)급현저성(P＜0．05)하강．가견,OP폭로대대하생장발육회산생영향．
The effects of octyl-phenol (OP)on N-acetylglucosiminidase (NAGase)activity in the epidermis and hepatopancreas were investigated after prawns (Litopenaeus vannamei)were exposured to the different octyl-phenol concentrations(0,5,15,45,135μg/L)for different times(24,48,72,96, 120 h),respectively.The results showed that OP could mainly activate NAGase in the epidermis.OP at the different concentrations had not significantly effect on the activity of the epidermal NAGase of prawns exposed for 72 hours,OP at 5,15 and 135μg/L extremely significantly activated and OP at 45μg/L significantly activated the epidermal NAGase of prawns exposed for 9 6 hours,respectively.After a 120 hours exposure to 15 and 135μg/L OP,there were still significant increases in the activity of the epidermal NAGase.However,OP could mainly inhibit the hepatopancreatic NAGase.After a 24 hours exposure to 1 5μg/L OP,there was extremely significant decrease in the activity of hepatopancreatic NAGase.OP at 5,15,45 and 135μg/L extremely significantly inhibited the hepatopancreatic NAGase of prawns exposed for 48 hours.OP at 15,45 and 135μg/L extremely significantly inhibited the hepa-topancreatic NAGase of prawns exposed for 72 hours.OP at 15 and 135μg/L extremely significantly and significantly inhibited the hepatopancreatic NAGase of prawns exposed for 9 6 hours,respectively.
