CAJ | 학술논문

通过水培法研究了不同Cu2＋浓度(0．5,1．0,2．5,5．0,10．0 mg/L)对水生植物凤眼莲[Eichhornia crassipes]蛋白质含量、根系活力及Cu2＋-ATP酶活性的影响．结果表明,Cu2＋胁迫对凤眼莲蛋白质含量、根系活力及Cu2＋-ATP酶活性的影响与 Cu2＋胁迫浓度及胁迫时间均有关,在 Cu2＋胁迫时间为1,3,10 d 时,各Cu2＋胁迫浓度下均有利于凤眼莲叶片蛋白质含量的积累．一定浓度和时间的 Cu2＋胁迫使凤眼莲体内蛋白质含量应激积累增多．在Cu2＋胁迫时间为1 d时,随着Cu2＋浓度的增加,凤眼莲根系活力逐渐增大；当胁迫时间达10 d时,根系活力在Cu2＋浓度为1．0 mg/L Cu2＋时达最大,以后逐渐降低．试验表明,随着 Cu2＋浓度的增加,叶片Cu2＋-ATP酶活性在0．5 mg/L时达最大,之后逐渐降低；随Cu2＋浓度的增加根系Cu2＋-ATP酶活性的变化趋势与叶片Cu2＋-ATP酶活性的变化趋势相似．
통과수배법연구료불동Cu2＋농도(0．5,1．0,2．5,5．0,10．0 mg/L)대수생식물봉안련[Eichhornia crassipes]단백질함량、근계활력급Cu2＋-ATP매활성적영향．결과표명,Cu2＋협박대봉안련단백질함량、근계활력급Cu2＋-ATP매활성적영향여 Cu2＋협박농도급협박시간균유관,재 Cu2＋협박시간위1,3,10 d 시,각Cu2＋협박농도하균유리우봉안련협편단백질함량적적루．일정농도화시간적 Cu2＋협박사봉안련체내단백질함량응격적루증다．재Cu2＋협박시간위1 d시,수착Cu2＋농도적증가,봉안련근계활력축점증대；당협박시간체10 d시,근계활력재Cu2＋농도위1．0 mg/L Cu2＋시체최대,이후축점강저．시험표명,수착 Cu2＋농도적증가,협편Cu2＋-ATP매활성재0．5 mg/L시체최대,지후축점강저；수Cu2＋농도적증가근계Cu2＋-ATP매활성적변화추세여협편Cu2＋-ATP매활성적변화추세상사．
Water culture method is adopted to research the effects of the stresses of Cu2+ with different concentrations(0.5,1.0,2.5,5.0,10.0 mg??L-1 )on activities of root activity ,protein content and Cu2+-ATPase in the roots and leaves of Eichhornia crassipes seedlings.The results show that the effects of the stresses of Cu2+ in short time and low concentrations of Cu2+ is conducive to the accu-mulation of the protein content in the leaves of Eichhornia crassipes.The effects of the stresses of Cu2+ in short time and high concentrations of Cu2+ is conducive to the accumulation of the protein content in the roots of Eichhornia crassipes and the effects of the stresses of Cu2+,in long time and low concentrations of Cu2+ is also conducive to the accumulation of the protein content in the roots of Eichhornia crassipes.With the stresses of Cu2+ in one day,the stress of Cu2+ can enhance the root ac-tivity with the enlargement of the stresses of Cu2+.While with the stresses of Cu2+ in ten days,the root activity reaches the maximum in 1 .0 mg??L-1 of the concentration of Cu2+and then gradually re-duces.Along with the increase of Cu2+ concentration,the Cu2+-ATPase activity of leave reaches the maximum in 0.5 mg??L-1 of the concentration of Cu2+and then gradually reduces.And the variation of Cu2+-ATPase activity of root is the same as the leave.It can be concluded that the effect of Cu2+ on root activity,protein content and Cu2+-ATPase in the roots of Eichhornia crassipes is related to con-centration of Cu2+ and stress time.