CAJ | 학술논문

利用微生物分离培养方法从互花米草根部和叶片获得内生细菌,经重金属筛选,分离到一种耐受重金属Cu2＋、Pb2＋和Cr6＋的细菌．为了进一步鉴定分离菌株,利用 PCR 方法扩增其部分16S rDNA 序列,目标DNA条带约1500 bp,测序和在线分析结果后显示,所测序列均与基因库中已上传的 Lysinibacillus sphaericus菌株16S rDNA 序列(KJ878614．1,JN613478．1)相似性均为100％．初步鉴定该菌为 Lysinibacillus属球形芽孢杆菌(Lysinibacillus sphaericus),命名为Lysinibacillus sphaericus QZ1-1．对该菌株采用促生长特性分析结果显示,该菌株具有产生 IAA和 ACC的能力,同时该菌能在无氮培养基中生长,具备固氮功能,为日后重金属污染生物修复相关研究提供了良好的目标菌株．
이용미생물분리배양방법종호화미초근부화협편획득내생세균,경중금속사선,분리도일충내수중금속Cu2＋、Pb2＋화Cr6＋적세균．위료진일보감정분리균주,이용 PCR 방법확증기부분16S rDNA 서렬,목표DNA조대약1500 bp,측서화재선분석결과후현시,소측서렬균여기인고중이상전적 Lysinibacillus sphaericus균주16S rDNA 서렬(KJ878614．1,JN613478．1)상사성균위100％．초보감정해균위 Lysinibacillus속구형아포간균(Lysinibacillus sphaericus),명명위Lysinibacillus sphaericus QZ1-1．대해균주채용촉생장특성분석결과현시,해균주구유산생 IAA화 ACC적능력,동시해균능재무담배양기중생장,구비고담공능,위일후중금속오염생물수복상관연구제공료량호적목표균주．
Endophytic bacteria were obtained from roots and leaves of Spartina alterniflora using cul-ture-dependent method.After heavy metal screening,a strain showed resistance to Cu2+,Pb2+ and Cr6+ was obtained.To further characterize the isolation,partial 16S rDNA with length 1 500 bp was amplificated and sequenced.Online analysis suggested that all sequences showed 100% sequence identi-ty to previously submitted strains Lysinibacillus sp.ES3-1 (KJ 878614.1)and Lysinibacillus sp.O-E16 (JN 613478.1).We infered the isolation belongs to Lysinibacillus sphaericus,named Lysinibacil-lus sphaericus QZ1-1 .Growth characteristic analysis indicated that this strain could produce IAA and ACC deaminase,grow in nitrogen-free medium,suggesting its ability of nitrogen fixation.This study indicated that Lysinibacillus sphaericus QZ1-1 is a strong candidate for further related studies because of its potential phytoremodiation of heavy metal pollution.