CAJ | 학술논문

采用RT－PCR方法从文心兰（ Oncidium ‘Sweet Sugar’）中克隆到一个PEBP家族基因，该基因命名为On?FT－like （登录号AHB86975）。该基因编码区531 bp，编码177个氨基酸。蛋白序列比对和进化树分析表明， OnFT－like蛋白与蝴蝶兰FT蛋白的相似性为99％，进化距离最近。 qRT－PCR分析表明，OnFT－like在根和花器官没有检测到表达；在苗期和花蕾期的叶片和花葶中有表达，其中在花蕾期花葶中的表达量最高；在盛花期和花后期的叶片和花葶中不表达。分析认为，OnFT－like基因可能在花期转变中起调控作用。将OnFT－like基因连接到pCAMBIA1301载体上，成功构建了正义和反义植物表达载体，为下一步进行文心兰OnFT－like基因功能鉴定及遗传改良奠定基础。
채용RT－PCR방법종문심란（ Oncidium ‘Sweet Sugar’）중극륭도일개PEBP가족기인，해기인명명위On?FT－like （등록호AHB86975）。해기인편마구531 bp，편마177개안기산。단백서렬비대화진화수분석표명， OnFT－like단백여호접란FT단백적상사성위99％，진화거리최근。 qRT－PCR분석표명，OnFT－like재근화화기관몰유검측도표체；재묘기화화뢰기적협편화화정중유표체，기중재화뢰기화정중적표체량최고；재성화기화화후기적협편화화정중불표체。분석인위，OnFT－like기인가능재화기전변중기조공작용。장OnFT－like기인련접도pCAMBIA1301재체상，성공구건료정의화반의식물표체재체，위하일보진행문심란OnFT－like기인공능감정급유전개량전정기출。
A PEBP family gene was isolated from the leaf of Oncidium‘Sweet Sugar’ by using RT-PCR approaches,the gene was named OnFT-like(The accession number in GenBank:AHB86975).The coding re?gion of this gene was 531 bp and it encoded 177 putative amino acid residues. Multiple sequence alignments and phylogenetic tree analyses showed that OnFT-like was close to FT from phalaenopsis hybrid with 99% se?quence similarity.The expression was analysed by real-time fluorescent quantitative PCR,the results showed that the expression of OnFT-like was not detected in roots and flower organs;the expression of OnFT-like was observed in both leaves and inflorescence rachis at the seedling stage and bud stage,and the highest amount of expression appeared at the bud stages;OnFT-like was not expressed in both leaves and inflorescence rachis at the full-bloom and post-flowering stages. OnFT-like was connected to pCAMBIA1301 vectors,the sense and antisense plant expression vectors were constructed successfully and can be used for research into the function of OnFT-like and genetic improvement in Oncidium.