CAJ | 학술논문

通过单因素与正交试验设计L9（34）研究了蚯蚓自身蛋白酶进行自溶水解蚯蚓蛋白的最佳工艺条件，并分析了酶解产物中氨基酸组成特征。结果表明：蚯蚓自身蛋白酶进行自溶水解蚯蚓蛋白的最佳工艺条件为pH 6．5、鲜蚯蚓与水重量比为1∶1．5、温度50℃、水解反应时间48 h，在此条件下，100 g鲜蚯蚓酶解的水解液中总氨基酸有10．83 g，其中18种游离必需氨基酸达8．92 g，酶解产物大部分主要由分子量5000以下的多肽、小分子肽及氨基酸组成，其中300分子量以下的占了88．44％，可见酶解产物中氨基酸含量丰富，组成平衡，具有进一步开发成氨基酸饲料添加剂及其它农用生化产品的潜力。
통과단인소여정교시험설계L9（34）연구료구인자신단백매진행자용수해구인단백적최가공예조건，병분석료매해산물중안기산조성특정。결과표명：구인자신단백매진행자용수해구인단백적최가공예조건위pH 6．5、선구인여수중량비위1∶1．5、온도50℃、수해반응시간48 h，재차조건하，100 g선구인매해적수해액중총안기산유10．83 g，기중18충유리필수안기산체8．92 g，매해산물대부분주요유분자량5000이하적다태、소분자태급안기산조성，기중300분자량이하적점료88．44％，가견매해산물중안기산함량봉부，조성평형，구유진일보개발성안기산사료첨가제급기타농용생화산품적잠력。
The optimum enzymatic hydrolysis process by autolytic protease in earthworm was studied through mono-factor analysis and orthogonal test L9(34).At the same time,the component characteristics of its enzymatic hydrolysates was determined.The results showed:the optimum conditions for the enzymatic hydrolysis process were pH value 7.0,temperature 50 ℃,reaction time 48 hours,the weight ratio of fresh earthworm to water 1 ∶ 1.5.Under such conditions,the content of 18 free necessary amino acids and total amino acid concen?tration of earthworm enzymatic hydrolysates could reach 8.92 g and 10.83 g per 100 g fresh earthworm,respec?tively.And the enzymatic hydrolysates of earthworm mainly consisted of polypeptides,small peptides and amino acids and their molecular weights were below 5 000,and the hydrolysis products with molecular weights below 300 took up 88.44% of all the enzymatic hydrolysates.Such results indicated that the types of essential amino acids were plenty and the components of amino acids were in balance in the earthworm enzymatic hydrolysates.Therefore,it suggested that the earthworm enzymatic hydrolysates had the potential to be used to manufacturenew feed additive of amino acid and other agricultural biochemical products.