CAJ | 학술논문

初步分离纯化了链霉菌JD211所产抗菌活性物质。结果显示：活性产物最易溶解在正丁醇中，其次为乙酸乙酯；微溶于氯仿；不溶于苯、乙醚和石油醚等低极性的有机溶剂。硅胶柱层析中用1∶5的石油醚：乙酸乙酯作为洗脱剂时的洗脱液具有最大的抑菌活性。薄层层析实验结果表明：以1∶5的石油醚：乙酸乙酯作为展开剂展开时，Rf值为0．53和0．6时具有抑菌活性。液相色谱－质谱联用分析表明活性物质与放线菌酮标准品具有相同的分子量，都为281．25。确定活性物质为戊二酰亚胺类抗生素。
초보분리순화료련매균JD211소산항균활성물질。결과현시：활성산물최역용해재정정순중，기차위을산을지；미용우록방；불용우분、을미화석유미등저겁성적유궤용제。규효주층석중용1∶5적석유미：을산을지작위세탈제시적세탈액구유최대적억균활성。박층층석실험결과표명：이1∶5적석유미：을산을지작위전개제전개시，Rf치위0．53화0．6시구유억균활성。액상색보－질보련용분석표명활성물질여방선균동표준품구유상동적분자량，도위281．25。학정활성물질위무이선아알류항생소。
The isolation,purification and identification of the antifungal active substances from Streptomy?ces JD211 were conducted.The results showed that the antifungal active substances were most inclined to dis?solve in n-butanol,followed by ethyl acetate;slightly soluble in chloroform;insoluble in benzene,ether and pe?troleum ether and other low-polar organic solvents.Column chromatography on silica gel using 1 ∶ 5 petroleum ether:ethyl acetate as the eluent agent had the greatest inhibitory activity.TLC results showed that 1 ∶ 5 petro?leum ether:ethyl acetate which were used as the eluent to start,when Rf values were 0.53 and 0.6,had anti?bacterial activity.Liquid chromatography-mass spectrometry analysis showed that the active substance and ac?tidione standard had the same molecular weight,both were 281.25.The active substance was determined as glu?tarimide antibiotics.