CAJ | 학술논문

旨在观察镉胁迫下钼对山羊肾线粒体抗氧化能力，以期探讨两者的毒理学关系。选用36头健康波尔山羊随机分成4组，每组9头，选用钼酸铵[（NH4）6Mo7O24·4H2O]、氯化镉（CdCl2）作为实验钼源、镉源。对照组（实验1组）灌服实验组平均剂量去离子水，实验2组、实验3组和实验4组灌服Cd 1 mg／kg（ BW）同时再分别灌服Mo 15 mg／kg（ BW）、30 mg／kg（ BW）和45 mg／kg（ BW），每组3个重复。实验期50 d，于实验第0、25、50天每组取肾组织，测定相关指标。结果表明：（1）与对照组相比，在实验第25、50天时，实验组山羊肾线粒体T－AOC、SOD活性降低（ P＜0．01），MDA、NO、NOS、CAT含量或活性升高（ P＜0．05），并随着时间天数的增加，肾线粒体T－AOC、SOD活性呈降低趋势（P＜0．01），而MDA、NO、NOS、CAT含量或活性呈升高趋势（P＜0．05）；（2）实验各组比较，当镉剂量在一定水平时，各实验组之间随着灌服钼剂量的升高， T－AOC、SOD 活性呈下降趋势， MDA、NO、NOS活性或含量呈上升趋势。钼镉可导致肾线粒体抗氧化功能降低、自由基含量升高，与镉呈协同关系。
지재관찰력협박하목대산양신선립체항양화능력，이기탐토량자적독이학관계。선용36두건강파이산양수궤분성4조，매조9두，선용목산안[（NH4）6Mo7O24·4H2O]、록화력（CdCl2）작위실험목원、력원。대조조（실험1조）관복실험조평균제량거리자수，실험2조、실험3조화실험4조관복Cd 1 mg／kg（ BW）동시재분별관복Mo 15 mg／kg（ BW）、30 mg／kg（ BW）화45 mg／kg（ BW），매조3개중복。실험기50 d，우실험제0、25、50천매조취신조직，측정상관지표。결과표명：（1）여대조조상비，재실험제25、50천시，실험조산양신선립체T－AOC、SOD활성강저（ P＜0．01），MDA、NO、NOS、CAT함량혹활성승고（ P＜0．05），병수착시간천수적증가，신선립체T－AOC、SOD활성정강저추세（P＜0．01），이MDA、NO、NOS、CAT함량혹활성정승고추세（P＜0．05）；（2）실험각조비교，당력제량재일정수평시，각실험조지간수착관복목제량적승고， T－AOC、SOD 활성정하강추세， MDA、NO、NOS활성혹함량정상승추세。목력가도치신선립체항양화공능강저、자유기함량승고，여력정협동관계。
This experiment was conducted to determine the effect of stress from the combination of molyb?denum and cadmium on free radicals of kidney in goats and explore relationship between their toxicology. 36 healthy goats were divided into the following four groups randomly,CdCl2and [(NH4)6Mo7O24·4H2O] were selected as the source of cadmium and molybdenum.The treatments consisted of the control group(no additional Mo and Cd) ,the experimental groups 2,3 and 4,which were drenched with 15 mg of Mo ×kg-1 ×BW、30 mg of Mo ×kg-1×BW and 45 mg of Mo ×kg-1×BW each day respectively,and all the experimental groups were drenched with 1 mg of Cd ×kg-1×BW each day as well.The experimental period was 50 days,renal tissue samples were obtained on the 0 day,the 25th day and the 50th day by killing 3 goats from each group respectively,then the related in?dexes were determined.The results were as follows:compared with the control group,the levels of MDA,NO,NOS in each group were significantly higher than those in the control group,while the levels of T-AOC,SODwere lower (P<0.05);in addition,as the experimental time elapsed,the levels of MDA,NO,NOS in each ex?perimental group showed an increasing trend and the levels of T-AOC,SOD showed an decreasing trend.In theexperimental groups (except control group):with the increasing of molybdenum dosage,the levels of T-AOC,T-SOD decreased while those of MDA,NO and NOS increased when cadmium was at the same level.(Conclu?sion) The results indicated that under the molybdenosis and cadmium poisoning in goat,cadmium and molyb?denum had a synergetic effect on kidney mitochondria antioxidant function.