CAJ | 학술논문

从健康仔猪的肠道中分离筛选到一株乳酸菌，经生理生化测定和16s rDNA基因序列同源性分析，该菌株为屎肠球菌，命名为WLEF－01。该菌株在MRS培养基37℃下培养，延滞期约0．5 h，对数生长期的代时为20 min，稳定期阶段的浓度可达1．5×1011cfu／mL。 WLEF－01的产酸能力较强，可使培养基pH从6．8降至4．5，并可耐受1％的猪胆盐作用2 h，在pH4以上酸性环境有耐受能力，并具有拮抗大肠杆菌和金黄色葡萄球菌生长的作用。在饲料中按2×107cfu／g浓度添加，饲喂泰和乌骨鸡12周龄，安全性好，有一定的益生功能。结果表明，屎肠球菌WLEF－01菌株具有作为益生菌饲料添加剂的开发应用潜力。
종건강자저적장도중분리사선도일주유산균，경생리생화측정화16s rDNA기인서렬동원성분석，해균주위시장구균，명명위WLEF－01。해균주재MRS배양기37℃하배양，연체기약0．5 h，대수생장기적대시위20 min，은정기계단적농도가체1．5×1011cfu／mL。 WLEF－01적산산능력교강，가사배양기pH종6．8강지4．5，병가내수1％적저담염작용2 h，재pH4이상산성배경유내수능력，병구유길항대장간균화금황색포도구균생장적작용。재사료중안2×107cfu／g농도첨가，사위태화오골계12주령，안전성호，유일정적익생공능。결과표명，시장구균WLEF－01균주구유작위익생균사료첨가제적개발응용잠력。
A strain of lactic acid bacterium was screened from the intestinal tract of a healthy piglet.It’s i?dentification was based on bacterial culture,physiological and biochemical tests and 16S rDNA sequence analy?sis.Its potential probiotic feature was evaluated by its growth curve in MRS and its functional properties,inclu?ding antagonistic activity against pathogenic bacteria,survival form gastrointestinal tract conditions ( acidity and bile),antibiotic susceptibility and safety in chicken feeding.A strain WLEF-01 was identified as Enterococcus faecium.It exhibited rapid growth in MRS at 37℃ and entered the logarithmic phase after incubation 0.5 h.The generation time of WLEF-01 was 20 min and it reached 1.5×1011cfu/ mL in the stationary phase.The strain se?creted acid to the medium making pH 6.8 to 4.5 and showed better resistance to 1% bile salted and lower pH4.WLEF-01 revealed antagonism against pathogenic E.Coli and S.aureus to some degree.Fed to chicken from 0to 12 weeks,WLEF-01 2×107cfu/ g had no bad effects,which suggested that the strain is safe.From the resultsobtained,it can be concluded that WLEF-01 isolated from piglet intestinal tract is a potential probiotic strainfor feeding additive.