CAJ | 학술논문

滞育是昆虫逃避不利环境的基本方式之一，但目前仅极少数基因被证明直接参与了昆虫滞育的调控。综述了昆虫滞育关联差异基因的筛选方法及相关方法的应用概况，包括差异显示反转录PCR、构建全长cDNA文库、基因表达序列标签、基因芯片技术和RNA转录组测序等在滞育差异表达基因筛选中的研究应用。对蛋白质组学和RNA干扰技术在昆虫滞育调控中关联蛋白和基因的功能研究一并进行了总结。为昆虫滞育调控机制中关键基因的发掘、滞育进程中对逆境条件耐受机制和探索害虫生态调控的新途径提供参考。
체육시곤충도피불리배경적기본방식지일，단목전부겁소수기인피증명직접삼여료곤충체육적조공。종술료곤충체육관련차이기인적사선방법급상관방법적응용개황，포괄차이현시반전록PCR、구건전장cDNA문고、기인표체서렬표첨、기인심편기술화RNA전록조측서등재체육차이표체기인사선중적연구응용。대단백질조학화RNA간우기술재곤충체육조공중관련단백화기인적공능연구일병진행료총결。위곤충체육조공궤제중관건기인적발굴、체육진정중대역경조건내수궤제화탐색해충생태조공적신도경제공삼고。
Diapause is one of the basicmodes for insects to escape from unfavorable environment,however, there are very few genes which are proved to be involved in the regulation of diapause.This paper summarizes the methods for screening differential expression genes related to insect diapause including display reverse tran?scription PCR,construction of full length cDNA library,expressed sequence tags,microarray and RNA-Se?quencing technology,which have been used in screening differentially expressed genes related to insect dia?pause.The proteomics and RNA interference technology used to exploring the function of key proteins and genes associated with the regulation of insect diapause are also summarized.This review may provide some valu?able reference for exploring key genes in insect diapause regulating mechanism,understanding the mechanisms of diapausing insect response to reverse environmental conditions and exploring new ways for ecological pest management.