CAJ | 학술논문

目的：探讨微小 RNA（microRNA，miRNA）异常表达对肥胖儿童代谢综合征发生发展的影响。方法应用 miR－NA 表达谱芯片检测肥胖合并代谢综合征患儿、单纯性肥胖患儿及正常体重儿童血清中 miRNA 的表达谱；利用实时定量 PCR技术检测其差异表达的 miRNA，验证 miRNA 芯片结果的可靠性；应用软件对筛选出的显著差异表达 miRNA 的靶基因进行预测。结果单纯性肥胖患儿血清中，上调表达超过1．5倍的 miRNA 有15个，下调表达超过1．5倍的有11个。肥胖合并代谢综合征患儿血清中9个上调 miRNA，7个下凋 miRNA。其中肥胖合并代谢综合征患儿 hsa－miR－143、hsa－miR－24、hsa－miR－34a和 has－miR－29a 表达高于单纯性肥胖患儿，下调表达中有 hsa－miR－192和 hsa－miR－23a 表达低于单纯性肥胖儿童。通过生物信息学分析发现了表达显著改变 miRNA 的共同作用的靶基因。结论筛选出肥胖合并代谢综合征患儿的差异表达 miRNA，可能参与其对应共同靶基因，具有调控肥胖儿童合并代谢综合征发生发展的作用。
목적：탐토미소 RNA（microRNA，miRNA）이상표체대비반인동대사종합정발생발전적영향。방법응용 miR－NA 표체보심편검측비반합병대사종합정환인、단순성비반환인급정상체중인동혈청중 miRNA 적표체보；이용실시정량 PCR기술검측기차이표체적 miRNA，험증 miRNA 심편결과적가고성；응용연건대사선출적현저차이표체 miRNA 적파기인진행예측。결과단순성비반환인혈청중，상조표체초과1．5배적 miRNA 유15개，하조표체초과1．5배적유11개。비반합병대사종합정환인혈청중9개상조 miRNA，7개하조 miRNA。기중비반합병대사종합정환인 hsa－miR－143、hsa－miR－24、hsa－miR－34a화 has－miR－29a 표체고우단순성비반환인，하조표체중유 hsa－miR－192화 hsa－miR－23a 표체저우단순성비반인동。통과생물신식학분석발현료표체현저개변 miRNA 적공동작용적파기인。결론사선출비반합병대사종합정환인적차이표체 miRNA，가능삼여기대응공동파기인，구유조공비반인동합병대사종합정발생발전적작용。
Objective To discuss influence of abnormal expression of miRNA on the cause and development of metabolic syn -drome in childhood obesity.Methods MiRNA microarray was used to detect the differential expression of miRNA in blood serum of met -abolic syndrome in childhood obesity,childhood obesity only and the healthy children .Real-time quantitative reverse transcription -poly-merase chain reaction(RT-PCR) was performed to verify the differential expression of candidate miRNA obtained from microarray experi -ment.Bioinformatic software tools were used to predict the target genes of identified miRNA .Results Fifteen miRNAs were upregulated more than 1.5-fold,and 11 miRNAs were downregulated in childhood obesity .Nine miRNAs were upregulated more than 1.5-fold,7 miR-NAs were downregulated in metabolic syndrome in childhood obesity .The expression of hsa-miR-143,hsa-miR-24,hsa-miR-34a,and has-miR-29a of the metabolic syndrome group were upregulated more than childhood obesity only group ,hsa-miR-192 and hsa-miR-23a were downregulated.The results of real-time quantitative RT-PCR were consistent with those of miRNA microarray.The common miRNA targets of obviously upregulated miRNAs and downregulated miRNAs were discovered.Conclusion Some miRNAs are differentially expressed and may play an important role in common target genes in regulation of occurrence and development of Metabolic syndrome in childhood obesity.