CAJ | 학술논문

目的：目前精神分裂症的临床诊断缺乏统一的标准。文中研究精神分裂症患者血清中差异miRNA表达谱，为该病的临床诊断提供依据。方法采用FlashTag TM Biotin RNA芯片技术和SAM软件筛选精神分裂症患者与健康受试者血清中差异表达的miRNA，用实时荧光定量逆转录PCR（RT-PCR）进行验证。结果筛选出3个差异表达的miRNA，其中hsa-miR-1281上调，表达倍数变化为1．50881；hsa-miR-2861、hsa-miR-638下调，表达倍数变化分别为0．64193和0．51624。实时定量PCR结果证实，精神分裂症患者较健康受试者hsa-miR-2861与hsa-miR-638表达下调，分别为[（0．037±0．037） vs （3．159±2．761），χ2＝4．089， P＜0．05]及[（0．006±0．006） vs （0．689±0．314），χ2＝4．083， P＜0．05]，hsa-miR-1281在精神分裂症患者组呈高表达[（1．221±0．041） vs （0．145±0．036），χ2＝5．333，P＜0．05）。结论精神分裂症患者血清中存在差异表达的miRNA谱，miR-1281、miR-2861和miR-638可作为诊断精神分裂症的一个新的血清标志物。
목적：목전정신분렬증적림상진단결핍통일적표준。문중연구정신분렬증환자혈청중차이miRNA표체보，위해병적림상진단제공의거。방법채용FlashTag TM Biotin RNA심편기술화SAM연건사선정신분렬증환자여건강수시자혈청중차이표체적miRNA，용실시형광정량역전록PCR（RT-PCR）진행험증。결과사선출3개차이표체적miRNA，기중hsa-miR-1281상조，표체배수변화위1．50881；hsa-miR-2861、hsa-miR-638하조，표체배수변화분별위0．64193화0．51624。실시정량PCR결과증실，정신분렬증환자교건강수시자hsa-miR-2861여hsa-miR-638표체하조，분별위[（0．037±0．037） vs （3．159±2．761），χ2＝4．089， P＜0．05]급[（0．006±0．006） vs （0．689±0．314），χ2＝4．083， P＜0．05]，hsa-miR-1281재정신분렬증환자조정고표체[（1．221±0．041） vs （0．145±0．036），χ2＝5．333，P＜0．05）。결론정신분렬증환자혈청중존재차이표체적miRNA보，miR-1281、miR-2861화miR-638가작위진단정신분렬증적일개신적혈청표지물。
Objective No uniform standards has been established for the clinical diagnosis of schizophrenia .The article was to investigate the differentially expressed microRNA ( miRNA) profile and explore its clinical significance . Methods Differentially expressed miRNAs were screened by FlashTag TM Biotin RNA chips and SAM software in serum of patients with schizophrenia and healthy controls , then the validation was performed by real-time fluorescent quantitative reverse transcription polymerase chain reaction ( RT-PCR) . Results Three differentially expressed miRNAs were screened , including up-regulated hsa-miR-1281 ( fold change =1.50881) and two down-regulated miRNAs:hsa-miR-2861(fold change=0.642) and hsa-miR-638 (fold change=0.516).The com-parative analysis of RT-PCR by SPSS 17.0 Kruskal Wallis H Test validated the expression levels of hsa-miR-2861 and hsa-miR-638 in patients with schizophrenia decreased significantly in comparison to healthy controls (χ2 =4.089,χ2 =4.083, P<0.001).While the expression level of hsa-miR-1281 in patients with schizophrenia was in higher expression level compared with control group (χ2 =5.333, P<0.001). Conclusion There are differentially expressed miRNA profile in serum of patients with schizophrenia , in which miR-1281 , miR-2861 and miR-638 could be new serum markers for diagnosis of schizophrenia .