CAJ | 학술논문

目的：研究表明，Wnt信号通路与肾癌发生、发展密切相关。文中拟通过观察小分子Wnt信号抑制剂15-氧代绣线菊内酯对人肾癌786-0细胞增殖、迁移、细胞凋亡和周期等恶性表型的影响，探讨15-氧代绣线菊内酯对肾癌细胞的体外抗肿瘤效应。方法实验分为：低、中、高浓度15-氧代绣线菊内酯组（终浓度分别为1．25、2．50和5．00μmol／L的15-氧代绣线菊内酯）及对照组（DMSO），各组分别处理786-0细胞株，分别运用四甲基偶氮唑蓝（MTT）比色法和划痕损伤实验分别观察细胞增殖、迁移的变化，采用Annexin V-FITC／PI双染色及PI染色流式细胞术检测细胞凋亡及周期的变化情况。结果15-氧代绣线菊内酯能明显抑制肾癌786-0细胞株的增殖，半数抑制浓度为1．088μmol／L，降低细胞迁移距离（P＜0．05）；低、中、高浓度15-氧代绣线菊内酯组处理786-0细胞36 h 后，细胞凋亡率[（12．17±0．56）％、（18．54±1．07）％、（50．74±1．28）％]较对照组[（7．85±0．42）％]明显升高（P＜0．05），且随药物浓度增加，细胞凋亡率逐步升高。15-氧代绣线菊内酯能明显增加G 2／M期的细胞，减少G0／G1期的细胞（P＜0．05）。结论15-氧代绣线菊内酯在体外细胞学水平能够显著抑制786-0细胞的增殖和迁移，诱导细胞凋亡，可能是一种潜在的抗肿瘤药物。
목적：연구표명，Wnt신호통로여신암발생、발전밀절상관。문중의통과관찰소분자Wnt신호억제제15-양대수선국내지대인신암786-0세포증식、천이、세포조망화주기등악성표형적영향，탐토15-양대수선국내지대신암세포적체외항종류효응。방법실험분위：저、중、고농도15-양대수선국내지조（종농도분별위1．25、2．50화5．00μmol／L적15-양대수선국내지）급대조조（DMSO），각조분별처리786-0세포주，분별운용사갑기우담서람（MTT）비색법화화흔손상실험분별관찰세포증식、천이적변화，채용Annexin V-FITC／PI쌍염색급PI염색류식세포술검측세포조망급주기적변화정황。결과15-양대수선국내지능명현억제신암786-0세포주적증식，반수억제농도위1．088μmol／L，강저세포천이거리（P＜0．05）；저、중、고농도15-양대수선국내지조처리786-0세포36 h 후，세포조망솔[（12．17±0．56）％、（18．54±1．07）％、（50．74±1．28）％]교대조조[（7．85±0．42）％]명현승고（P＜0．05），차수약물농도증가，세포조망솔축보승고。15-양대수선국내지능명현증가G 2／M기적세포，감소G0／G1기적세포（P＜0．05）。결론15-양대수선국내지재체외세포학수평능구현저억제786-0세포적증식화천이，유도세포조망，가능시일충잠재적항종류약물。
Objective Wnt signaling plays an important role in the development and progression of renal cell carcinoma (RCC).This study aimed to evaluate the effects of the Wnt signaling inhibitor 15-oxospiramilactone on the proliferation , migration, cell apoptosis, and cycles of the human RCC cell line 786-0, and to investigate the possible mechanisms of this small molecule acting on RCC in ivtro. Methods We treated 786-0 cells with DMSO ( blank control group ) and 15-oxospiramilactone at the concentrations of1.25μmol/L (low 15 -OSL), 2.5μmol/L (medium 15-OSL), and 5μmol/L (high 15-OSL), respectively, for 72 hours.Then we observed the changes in the proliferation and migration of the 786-0 cells by MTT and scratch-wound assay and determined their apopto-sis and cycles by Annexin V-FITC/PI assay and flow cytometry . Results 15-oxospiramilactone significantly inhibited the growth of the 7860-cells, with the IC 50of 1.088 μmol/L at 72 hours, and decreased their migration distance (P<0.05).After 36 hours of treatment, the apoptosis rates of the 786-0 cells in the low, medium, and high 15-OSL groups were (12.17 ±0.56), (18.54 ± 1.07), and (50.74 ±1.28) %, respectively, significantly increased as compared with (7.85 ±0.42) %in the blank control group (P<0.05), and in an obviously concentration-dependent manner.15-oxospiramilactone remarkably reduced the number of cells in the G0/G1 phase and increased that in the G 2/M phase (P<0.05). Conclusion 15-oxospiramilactone can significantly inhibit the pro -liferation and migration and induce the apoptosis of 786-0cells in vitro.It may be a potential anti-RCC agent.