CAJ | 학술논문

目的：评估3．5代聚酰胺－胺树枝状分子（PAMAM）对牙本质小管的阻塞能力。方法：收集32个完整离体第三磨牙制备32个脱矿牙本质模型，并将其随机分为A、B、C、D 4组（n＝8），分别置于3．5代PAMAM溶液中处理0、1、30、60 min后，再同时浸泡于人工唾液中再矿化2周，然后分别用傅里叶变换红外分光镜（ FT－IR）、场发射扫描电镜（ FE－SEM）、能谱仪（ EDS）观察检测。结果：A组牙本质小管完全开放；B、C、D各组经PAMMA处理相应时间后，其大部分牙本质小管口均被矿化物封闭，B、C、D组牙本质小管封闭深度分别为（26．31±2．45）、（25．34±2．57）、（30．36±2．15）μm ，3组间差异无统计学意义（ P＞0．05）；A、B、C、D 4组钙磷比值两两间差异亦均无统计学意义（ P＞0．05）。结论：3．5代聚酰胺－胺对脱矿牙本质小管有一定的封闭作用，但其作用时间与牙本质小管的封闭效果无明显相关性。
목적：평고3．5대취선알－알수지상분자（PAMAM）대아본질소관적조새능력。방법：수집32개완정리체제삼마아제비32개탈광아본질모형，병장기수궤분위A、B、C、D 4조（n＝8），분별치우3．5대PAMAM용액중처리0、1、30、60 min후，재동시침포우인공타액중재광화2주，연후분별용부리협변환홍외분광경（ FT－IR）、장발사소묘전경（ FE－SEM）、능보의（ EDS）관찰검측。결과：A조아본질소관완전개방；B、C、D각조경PAMMA처리상응시간후，기대부분아본질소관구균피광화물봉폐，B、C、D조아본질소관봉폐심도분별위（26．31±2．45）、（25．34±2．57）、（30．36±2．15）μm ，3조간차이무통계학의의（ P＞0．05）；A、B、C、D 4조개린비치량량간차이역균무통계학의의（ P＞0．05）。결론：3．5대취선알－알대탈광아본질소관유일정적봉폐작용，단기작용시간여아본질소관적봉폐효과무명현상관성。
AIM: To evaluate the occluding ability of the 3.5 th generation of polyamidoamine dendrimer (PAMAM) on human dentinal tubules.METHODS:32 extracted human third molars were used to make sensitive dentin models and were divided into 4 groups (A, B, C and D) (n=8)randomly.The dentin models were treated with G3.5 PAMAM for 0(A), 1(B), 30(C) and 60(D) min respectively and subsequently immersed into artificial saliva for 2 weeks.The dentin discs were observed by Fouier Transform Infrared Spectroscopy ( FT-IR) , field emission scanning electron microscope ( FE-SEM) and energy dispersive spectrometer ( EDS) respectively.RESULTS:The oc-cluding depth (μm) in group B, C and D were 26.31 ±2.45, 25.34 ±2.57 and 30.36 ±2.15 respectively (P>0.05).The dentinal tubules in group A were all open.The Ca/P ratios were no significantly different among 4 groups (P>0.05).CONCLUTION:3.5 th generation of polyamidoamine dendrimer has occlusion effect on sensi-tive dentinal tubules.However, there was no significant correlation between its action time and the occluding capability.