牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
CHINESE JOURNAL OF CONSERVATIVE DENTISTRY
2014年
12期
701-704
,共4页
于西佼%杜言梅%姜欢%杜毅
于西佼%杜言梅%薑歡%杜毅
우서교%두언매%강환%두의
上皮根鞘( HERS)%细胞牙骨质%细胞角蛋白14%发育
上皮根鞘( HERS)%細胞牙骨質%細胞角蛋白14%髮育
상피근초( HERS)%세포아골질%세포각단백14%발육
hertwig's epithelial root sheath( HERS)%cellular cementum%CK14%development
目的：通过追踪观察小鼠细胞牙骨质发育过程中的组织形态和细胞活性改变，探讨源于上皮根鞘断裂的上皮性细胞是否参与细胞牙骨质的发育。方法：选取出生后15、19、25 d BALB／c小鼠的下颌第一磨牙根尖1／3区细胞牙骨质，细胞角蛋白14（ CK 14）标记追踪上皮根鞘断裂后的上皮性细胞；TUNEL （ TdT－mediated－dUTP nick end labeling ， TUNEL）法检测细胞牙骨质在发育过程中的细胞凋亡情况；透射电镜观察细胞牙骨质发育的超微结构。结果：细胞凋亡检测结果显示，细胞牙骨质形成过程中可见大量被埋入或正被埋入细胞呈现凋亡阳性表达；免疫组化染色结果显示，其中部分细胞呈CK14阳性表达；透射电镜观察结果显示，细胞牙骨质形成过程中上皮性细胞被其外侧成牙骨质细胞分泌的基质包围。结论：来源上皮根鞘断裂的上皮性细胞可能作为牙骨质细胞参与细胞牙骨质的形成。
目的：通過追蹤觀察小鼠細胞牙骨質髮育過程中的組織形態和細胞活性改變，探討源于上皮根鞘斷裂的上皮性細胞是否參與細胞牙骨質的髮育。方法：選取齣生後15、19、25 d BALB／c小鼠的下頜第一磨牙根尖1／3區細胞牙骨質，細胞角蛋白14（ CK 14）標記追蹤上皮根鞘斷裂後的上皮性細胞；TUNEL （ TdT－mediated－dUTP nick end labeling ， TUNEL）法檢測細胞牙骨質在髮育過程中的細胞凋亡情況；透射電鏡觀察細胞牙骨質髮育的超微結構。結果：細胞凋亡檢測結果顯示，細胞牙骨質形成過程中可見大量被埋入或正被埋入細胞呈現凋亡暘性錶達；免疫組化染色結果顯示，其中部分細胞呈CK14暘性錶達；透射電鏡觀察結果顯示，細胞牙骨質形成過程中上皮性細胞被其外側成牙骨質細胞分泌的基質包圍。結論：來源上皮根鞘斷裂的上皮性細胞可能作為牙骨質細胞參與細胞牙骨質的形成。
목적：통과추종관찰소서세포아골질발육과정중적조직형태화세포활성개변，탐토원우상피근초단렬적상피성세포시부삼여세포아골질적발육。방법：선취출생후15、19、25 d BALB／c소서적하합제일마아근첨1／3구세포아골질，세포각단백14（ CK 14）표기추종상피근초단렬후적상피성세포；TUNEL （ TdT－mediated－dUTP nick end labeling ， TUNEL）법검측세포아골질재발육과정중적세포조망정황；투사전경관찰세포아골질발육적초미결구。결과：세포조망검측결과현시，세포아골질형성과정중가견대량피매입혹정피매입세포정현조망양성표체；면역조화염색결과현시，기중부분세포정CK14양성표체；투사전경관찰결과현시，세포아골질형성과정중상피성세포피기외측성아골질세포분비적기질포위。결론：래원상피근초단렬적상피성세포가능작위아골질세포삼여세포아골질적형성。
AIM:To observe the histological and cellular activity changes during cellular cementum develop-ment in BALB/c mice.METHODS:Post-natal 15, 19 and 25d BALB/c mice (8 in each group) were sacrificed and cellular cementum of mandibular first molar was taken.Cytokeratin 14 ( CK14 ) expression in epithelial cells of Hertwig′s epithelial root sheath ( HERS) was examined by PV two-step immunohistological method.The morphology and distribution of epithelial cells and cellular cementum were examined by light and transmission electron microscope ( TEM) .Apoptosis was identified by the terminal deoxy-transferase ( TdT)-mediated dUTP-biotin nick end labeling ( TUNEL) method.RESULTS:During cellular cementogenesis, some cells were incorporated in the cementum and positive CK14 expression can be observed.Following the disintegration of HERS, cementum matrix was rapidly deposi-ted around cementoblasts.Epithelial cells and cementoblasts on the root surface were embedded as cementocytes. CONCLUSION:Epithelial cells from disintegrated HERS may directly participate in cellular cementogenesis.
