CAJ | 학술논문

目的：观察不同浓度的五倍子水提取物对人牙髓细胞碱性磷酸酶（ ALP）活性及矿化能力的影响。方法：组织块酶消化法体外培养人牙髓细胞，并分别与不同浓度的五倍子水提取物（2．5、5、10、20、40μg／mL）共同培养；于培养48 h后，采用酶组织化学法检测各组细胞的ALP活性。并根据ALP实验结果筛选出（10、20μg／mL）五倍子水提取物浓度组，与牙髓细胞共同培养30 d后，用Von Kossa染色法观察各组细胞的生长及矿化结节形成情况。结果：与对照组相比，2．5、5μg／mL的五倍子水提取物对人牙髓细胞的ALP活性无显著性影响（ P＞0．05），而浓度为10、20、40μg／mL的五倍子水提取物均可明显抑制牙髓细胞的ALP活性（P＜0．05）；Von Kossa染色结果显示，五倍子水提取物与人牙髓细胞共同培养30 d后，可促进其矿化结节的形成。结论：五倍子水提取物可在一定程度上改变人牙髓细胞的碱性磷酸酶活性及矿化程度。
목적：관찰불동농도적오배자수제취물대인아수세포감성린산매（ ALP）활성급광화능력적영향。방법：조직괴매소화법체외배양인아수세포，병분별여불동농도적오배자수제취물（2．5、5、10、20、40μg／mL）공동배양；우배양48 h후，채용매조직화학법검측각조세포적ALP활성。병근거ALP실험결과사선출（10、20μg／mL）오배자수제취물농도조，여아수세포공동배양30 d후，용Von Kossa염색법관찰각조세포적생장급광화결절형성정황。결과：여대조조상비，2．5、5μg／mL적오배자수제취물대인아수세포적ALP활성무현저성영향（ P＞0．05），이농도위10、20、40μg／mL적오배자수제취물균가명현억제아수세포적ALP활성（P＜0．05）；Von Kossa염색결과현시，오배자수제취물여인아수세포공동배양30 d후，가촉진기광화결절적형성。결론：오배자수제취물가재일정정도상개변인아수세포적감성린산매활성급광화정도。
AIM:To study the effects of gallnut water extraction on the alkaline phosphatase( ALP) activity and mineralization of human dental pulp cells( hDPCs) .METHODS:hDPCs were cultured with gallnut water extrac-tion at 2.5,5,10, 20 and 40μg/mL respectively.ALP expression of the cells was examined by immunocytochemistry after 48 hour culture.According to the ALP expression, the cells were treated by gallnut water extraction at 10μg/mL and 20μg/mL respectively for 30 days and Von Kossa staining was performed to detect mineralization.RESULTS:The extraction at 2.5μg/mL and 5μg/mL had no significant effect on ALP activity of the cells, while at 10, 20 and 40μg/mL inhibited ALP expression of the cells.Both 10μg/mL and 20μg/mL gallnut water extraction could promote mineralization of hDPCs after continuous cultivation for 30 days.CONCLUSION:Gallnut water extraction can change the ALP activity and mineralization of human dental pulp cells.