CAJ | 학술논문

目的：比较大鼠不同直径牙胚细胞增殖和骨向分化能力的差异。方法：酶消化法分离、培养SD大鼠牙胚细胞，经13μm标准细胞筛分选后获得大、小2种直径的细胞；并分别采用MTT法检测2种细胞的增殖能力；碱性磷酸酶（ ALP）试剂盒、茜素红染色法及qRT－PCR检测两者的骨向分化能力。结果：小细胞的增殖能力较低（P＜0．05）；培养3、7 d后，小细胞组的ALP活性均明显高于大细胞组（P＜0．05）；培养14 d后，小细胞组钙化结节数量明显高于大细胞组；qRT－PCR检测结果显示，小细胞组中Ocn、Osx、Runx2各矿化相关基因的表达水平均明显高于大细胞组（P＜0．05）。结论：牙胚细胞中直径小于13μm 的细胞比直径大于13μm的细胞增殖活性低，分化能力强。
목적：비교대서불동직경아배세포증식화골향분화능력적차이。방법：매소화법분리、배양SD대서아배세포，경13μm표준세포사분선후획득대、소2충직경적세포；병분별채용MTT법검측2충세포적증식능력；감성린산매（ ALP）시제합、천소홍염색법급qRT－PCR검측량자적골향분화능력。결과：소세포적증식능력교저（P＜0．05）；배양3、7 d후，소세포조적ALP활성균명현고우대세포조（P＜0．05）；배양14 d후，소세포조개화결절수량명현고우대세포조；qRT－PCR검측결과현시，소세포조중Ocn、Osx、Runx2각광화상관기인적표체수평균명현고우대세포조（P＜0．05）。결론：아배세포중직경소우13μm 적세포비직경대우13μm적세포증식활성저，분화능력강。
AIM: To compare the proliferation and osteogenic differentiation of rat tooth germ cells ( rT-GCs) with different diameters.METHODS:rTGCs were isolated enzymatically from Sprague-Dawley rats and divided into larger and smaller populations using a 13 μm cell sieve.MTT assay was performed to evaluate the cell prolifera-tion.Alkaline phosphotase ( ALP) kits, alizarin red staining and quantitative PCR were used to investigate the osteo-genic differentiation capacity of the cells.RESULTS: The larger rTGCs( rTGCs-Large) showed greater proliferation rate than the smaller counterparts ( rTGCs-Small) .However, rTGCs-Small group presented higher ALP activity at 3 and 7 d after culture and more calcified nodules than rTGCs-Large group at 14 d after culture.Moreover, rTGCs-Small group expressed stronger osteogenic markers Ocn, Osx and Runx2 than rTGCs-Large group.CONCLUSION:rTGCs-Small have lower proliferation capacity but stronger osteogenic differentiation potential than rTGCs-Large group.