东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2014年
12期
36-41
,共6页
H2A变体%PGCs%生殖嵴%分布
H2A變體%PGCs%生殖嵴%分佈
H2A변체%PGCs%생식척%분포
H2A variants%PGCs%genital ridge%distribution
小鼠原始生殖细胞进入生殖嵴之前经历细胞迁移、大量繁殖。为进一步解分子调控机制，文章通过组织石蜡切片免疫荧光染色和单细胞免疫荧光染色技术，对8.5、10.5 dpcPGCs中组蛋白H2A变体MacroH2A、H2A.Z、H2A. X的分布情况进行研究。结果表明，8.5~10.5 dpc，H2A.Z在PGCs中的表达明显递增，10.5 dpc PGCs中H2A.Z的表达主要集中于细胞核；而MacroH2A和H2A.X在此过程中无明显变化，在细胞质和细胞核中弱表达。综合分析发现，8.5~10.5 dpc PGCs中H2A.Z可能是装配核小体的主要变体，PGCs大量繁殖可能有H2A.Z的参与。
小鼠原始生殖細胞進入生殖嵴之前經歷細胞遷移、大量繁殖。為進一步解分子調控機製，文章通過組織石蠟切片免疫熒光染色和單細胞免疫熒光染色技術，對8.5、10.5 dpcPGCs中組蛋白H2A變體MacroH2A、H2A.Z、H2A. X的分佈情況進行研究。結果錶明，8.5~10.5 dpc，H2A.Z在PGCs中的錶達明顯遞增，10.5 dpc PGCs中H2A.Z的錶達主要集中于細胞覈；而MacroH2A和H2A.X在此過程中無明顯變化，在細胞質和細胞覈中弱錶達。綜閤分析髮現，8.5~10.5 dpc PGCs中H2A.Z可能是裝配覈小體的主要變體，PGCs大量繁殖可能有H2A.Z的參與。
소서원시생식세포진입생식척지전경력세포천이、대량번식。위진일보해분자조공궤제，문장통과조직석사절편면역형광염색화단세포면역형광염색기술，대8.5、10.5 dpcPGCs중조단백H2A변체MacroH2A、H2A.Z、H2A. X적분포정황진행연구。결과표명，8.5~10.5 dpc，H2A.Z재PGCs중적표체명현체증，10.5 dpc PGCs중H2A.Z적표체주요집중우세포핵；이MacroH2A화H2A.X재차과정중무명현변화，재세포질화세포핵중약표체。종합분석발현，8.5~10.5 dpc PGCs중H2A.Z가능시장배핵소체적주요변체，PGCs대량번식가능유H2A.Z적삼여。
Before the mouse primordial germ cells into the genital ridge through cell migration, proliferation, in order to further understand the molecular regulation mechanism. In this paper, through the organization of the paraffin section immunity fluorescence dyeing and single cell immunofluo-rescence staining technique, the distribution of 8.5, 10.5 dpcPGCs of histone H2A variants MacroH2A, H2A.Z, H2A.X were studied. The results showed that, 8.5-10.5 dpc, the expression of H2A.Z in PGCs was significantly increased, expression of H2A.Z 10.5 dpc in PGCs mainly in the nucleus; but no obvious changes of MacroH2A and H2A.X in the process, expressed in the cytoplasm and nucleus of weak. Comprehensive analysis showed that, 8.5-10.5 dpc H2A.Z in PGCs may be the main variant assembly of nucleosomes, PGCs during blooms may participate in the H2A.Z.
