CAJ | 학술논문

研究黄连解毒汤（HLJDT）对肿瘤生长的抑制作用及其对肿瘤组织COX-2、VEGF、bFGF的影响。将健康雌性昆明种小鼠50只（体重18~22 g）随机分为对照组，环磷酰胺组（CTX），黄连解毒汤大、中、小剂量组（22.0、11.0、5.5 g·kg-1）。通过皮下注射S180细胞悬液建立小鼠S180肉瘤模型，观察抑瘤率，并采用免疫组化的方法研究黄连解毒汤对肿瘤组织COX-2、VEGF、bFGF的作用。结果表明，黄连解毒汤大剂量组对小鼠S180肉瘤具有明显的抑制作用，免疫组化染色显示黄连解毒汤各剂量组对肿瘤组织中COX-2、bFGF表达有明显抑制作用，同时大、中剂量组对VEGF表达有明显抑制作用。说明黄连解毒汤对S180肉瘤具有抑制作用，其可抑制肿瘤组织中血管生成相关因子COX-2、VEGF、bFGF的表达，抑制肿瘤血管生长，抑制肿瘤生长，抗血管生成可能是其发挥治疗作用的另一种机制。
연구황련해독탕（HLJDT）대종류생장적억제작용급기대종류조직COX-2、VEGF、bFGF적영향。장건강자성곤명충소서50지（체중18~22 g）수궤분위대조조，배린선알조（CTX），황련해독탕대、중、소제량조（22.0、11.0、5.5 g·kg-1）。통과피하주사S180세포현액건립소서S180육류모형，관찰억류솔，병채용면역조화적방법연구황련해독탕대종류조직COX-2、VEGF、bFGF적작용。결과표명，황련해독탕대제량조대소서S180육류구유명현적억제작용，면역조화염색현시황련해독탕각제량조대종류조직중COX-2、bFGF표체유명현억제작용，동시대、중제량조대VEGF표체유명현억제작용。설명황련해독탕대S180육류구유억제작용，기가억제종류조직중혈관생성상관인자COX-2、VEGF、bFGF적표체，억제종류혈관생장，억제종류생장，항혈관생성가능시기발휘치료작용적령일충궤제。
The experiments were designed to study the effects of Huanglianjiedu Decoction(HLJDT) on antitumor activity in vivo and the expressions of COX-2, VEGF and bFGF in tumor tissues. 50 healthy female Kunming mice(18-22 g) were col ected, 50 tumor-bearing mice were randomly divided into NS control group, CTX positive group and 22.0, 11.0, 5.5 g·kg-1 HLJDT groups. We established S180 model of mice by transplanting 0.2 mL S180 cel suspension subcutaneously in mice, and immunohistochemical method was used to detect the expressions of COX-2, VEGF and bFGF.The results showed that the tumor-suppressing rate was higher in CTX group and HLJDT high dose group. COX-2 and bFGF in HLJDT groups and CTX group was lower than that in model group significantly. And the expressions of VEGF in HLJDT medium dose group and high dose group were markedly lower than that in model group. The results suggested that HLJDT had inhibitory effects on S180, and it also decreased the expression of COX-2, VEGF, bFGF in tumor tissue. Accordingly antiangiogenesis may be another mechanism for HLJDT to exert its treatment effects.