东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2014年
12期
1-9
,共9页
李一经%张博%唐丽杰%姜艳平%陈佩佩
李一經%張博%唐麗傑%薑豔平%陳珮珮
리일경%장박%당려걸%강염평%진패패
猪流行性腹泻病毒%遗传变异%S1基因%中和试验
豬流行性腹瀉病毒%遺傳變異%S1基因%中和試驗
저류행성복사병독%유전변이%S1기인%중화시험
PEDV%genetic variation%S1 gene%neutralization test
为探讨猪流行性腹泻(Porcine epidemic diarrhea,PED)再次爆发原因,对现阶段黑龙江省和内蒙古自治区的10株猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)流行毒株S1基因进行扩增、克隆和序列测定,通过序列比对分析其遗传演化特点。选择其中一株毒株进行中和试验,对其免疫原性做初步分析。结果表明,10株PEDV的S1基因与参考毒株相比,核苷酸同源性为86.8%~99.7%;且S1基因均存在点突变、碱基插入及缺失的情况;构建的系统发育树表明PEDV毒株在S1基因水平上共分为两个群,本试验中的10株均处在G1群,与2011~2012年3株其他地区毒株CH/SDQD/2011、CH/HBQHD/2011和HuN亲缘关系较近,而与G2群中的经典毒株CV777及我国以往报道过的LJB/03等亲缘关系较远;通过中和试验,判断出新发毒株HLJ-2012对传统毒株LJB/03具有一定的中和效应,中和效价为1??53.83。
為探討豬流行性腹瀉(Porcine epidemic diarrhea,PED)再次爆髮原因,對現階段黑龍江省和內矇古自治區的10株豬流行性腹瀉病毒(Porcine epidemic diarrhea virus,PEDV)流行毒株S1基因進行擴增、剋隆和序列測定,通過序列比對分析其遺傳縯化特點。選擇其中一株毒株進行中和試驗,對其免疫原性做初步分析。結果錶明,10株PEDV的S1基因與參攷毒株相比,覈苷痠同源性為86.8%~99.7%;且S1基因均存在點突變、堿基插入及缺失的情況;構建的繫統髮育樹錶明PEDV毒株在S1基因水平上共分為兩箇群,本試驗中的10株均處在G1群,與2011~2012年3株其他地區毒株CH/SDQD/2011、CH/HBQHD/2011和HuN親緣關繫較近,而與G2群中的經典毒株CV777及我國以往報道過的LJB/03等親緣關繫較遠;通過中和試驗,判斷齣新髮毒株HLJ-2012對傳統毒株LJB/03具有一定的中和效應,中和效價為1??53.83。
위탐토저류행성복사(Porcine epidemic diarrhea,PED)재차폭발원인,대현계단흑룡강성화내몽고자치구적10주저류행성복사병독(Porcine epidemic diarrhea virus,PEDV)류행독주S1기인진행확증、극륭화서렬측정,통과서렬비대분석기유전연화특점。선택기중일주독주진행중화시험,대기면역원성주초보분석。결과표명,10주PEDV적S1기인여삼고독주상비,핵감산동원성위86.8%~99.7%;차S1기인균존재점돌변、감기삽입급결실적정황;구건적계통발육수표명PEDV독주재S1기인수평상공분위량개군,본시험중적10주균처재G1군,여2011~2012년3주기타지구독주CH/SDQD/2011、CH/HBQHD/2011화HuN친연관계교근,이여G2군중적경전독주CV777급아국이왕보도과적LJB/03등친연관계교원;통과중화시험,판단출신발독주HLJ-2012대전통독주LJB/03구유일정적중화효응,중화효개위1??53.83。
To explore the possible reason of the PEDV re-outbreaks, the genetic variation characteristic of PEDV S1 genes was analyzed, S1 genes of 10 PEDV strains collected from Heilong-jiang Province and Inner Mongolia Autonomous Region were amplified, cloned, sequenced and compared. To make a preliminary immunogenicity analysis, one of the PEDV strains was chosen to perform the neutralization test. It shows that the S1 gene nucleotide homologies of 10 PEDV strains is 86.8%-99.7%compared with the reference ones;and point mutations, insertion, deletion are all existed in S1 genes of the 10 PEDV strains. Phylogenetic tree showed that PEDV S1 genes are divided into two groups on the gene level, 10 isolates in this study are all at G1, and have a closely relationship with CH/SDQD/2011, CH/HBQHD/2011 and HuN. While they have a far relationship with classic CV777 and Chinese previous isolates LJB/03 etc. By neutralization test, the newborn strain HLJ-2012 has a neut-ralizing effect on traditional strain LJB/03, and the neutralization titer was 1??53.83.