中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2014年
6期
516-520
,共5页
苏淼%赵彩彦%张莉%于伟燕
囌淼%趙綵彥%張莉%于偉燕
소묘%조채언%장리%우위연
肝炎,乙型,慢性%多态性,单核苷酸%磷%阿德福韦酯
肝炎,乙型,慢性%多態性,單覈苷痠%燐%阿德福韋酯
간염,을형,만성%다태성,단핵감산%린%아덕복위지
Hepatitis B,chronic%Polymorphism,single nucleotide%Phosphorus%Adefovir dipivoxil
目的：探讨SLC34A1（rs6420094）和RGS14（rs4074995）单核苷酸多态性与长期服用阿德福韦酯治疗的慢性HBV感染者血磷浓度的相关性。方法选取2012年10月至2013年8月河北医科大学第三医院连续服用阿德福韦酯（10 mg／d）单药或联合治疗至少2年的慢性HBV感染者91例，其中低磷血症患者31例，血磷正常患者60例。应用聚合酶链反应-限制性片段长度多态性分析法测定两位点的基因型，应用χ2检验分析两位点等位基因分布频率与血磷浓度的关系。结果低磷血症组中，rs6420094基因A／A、A／G和G／G型的例数分别为13，13和5例，而血磷正常组上述基因型的例数分别为35，24和1例。此位点的等位基因A在血磷正常组出现的频率高于低磷血症组（78．3％∶62．9％），两组比较差异有统计学意义（χ2＝4．947，P＜0．05）。低磷血症组中，rs4074995基因A／A、A／G和G／G型的例数分别为2，11和18例，而血磷正常组上述基因型的例数分别为1，21和38例，等位基因分布频率在两组间差异无统计学意义（χ2＝0．625，P＞0．05）。结论 rs6420094多态性可能会影响长期服用阿德福韦酯治疗的慢性HBV感染者的血磷浓度。
目的：探討SLC34A1（rs6420094）和RGS14（rs4074995）單覈苷痠多態性與長期服用阿德福韋酯治療的慢性HBV感染者血燐濃度的相關性。方法選取2012年10月至2013年8月河北醫科大學第三醫院連續服用阿德福韋酯（10 mg／d）單藥或聯閤治療至少2年的慢性HBV感染者91例，其中低燐血癥患者31例，血燐正常患者60例。應用聚閤酶鏈反應-限製性片段長度多態性分析法測定兩位點的基因型，應用χ2檢驗分析兩位點等位基因分佈頻率與血燐濃度的關繫。結果低燐血癥組中，rs6420094基因A／A、A／G和G／G型的例數分彆為13，13和5例，而血燐正常組上述基因型的例數分彆為35，24和1例。此位點的等位基因A在血燐正常組齣現的頻率高于低燐血癥組（78．3％∶62．9％），兩組比較差異有統計學意義（χ2＝4．947，P＜0．05）。低燐血癥組中，rs4074995基因A／A、A／G和G／G型的例數分彆為2，11和18例，而血燐正常組上述基因型的例數分彆為1，21和38例，等位基因分佈頻率在兩組間差異無統計學意義（χ2＝0．625，P＞0．05）。結論 rs6420094多態性可能會影響長期服用阿德福韋酯治療的慢性HBV感染者的血燐濃度。
목적：탐토SLC34A1（rs6420094）화RGS14（rs4074995）단핵감산다태성여장기복용아덕복위지치료적만성HBV감염자혈린농도적상관성。방법선취2012년10월지2013년8월하북의과대학제삼의원련속복용아덕복위지（10 mg／d）단약혹연합치료지소2년적만성HBV감염자91례，기중저린혈증환자31례，혈린정상환자60례。응용취합매련반응-한제성편단장도다태성분석법측정량위점적기인형，응용χ2검험분석량위점등위기인분포빈솔여혈린농도적관계。결과저린혈증조중，rs6420094기인A／A、A／G화G／G형적례수분별위13，13화5례，이혈린정상조상술기인형적례수분별위35，24화1례。차위점적등위기인A재혈린정상조출현적빈솔고우저린혈증조（78．3％∶62．9％），량조비교차이유통계학의의（χ2＝4．947，P＜0．05）。저린혈증조중，rs4074995기인A／A、A／G화G／G형적례수분별위2，11화18례，이혈린정상조상술기인형적례수분별위1，21화38례，등위기인분포빈솔재량조간차이무통계학의의（χ2＝0．625，P＞0．05）。결론 rs6420094다태성가능회영향장기복용아덕복위지치료적만성HBV감염자적혈린농도。
Objective To investigate the association of SLC34A1 ( rs6420094 ) and RGS14 (rs4074995) polymorphisms with serum level of phosphorus in chronic hepatitis B virus (HBV) infection patients following long-term adefovir dipivoxil ( ADV ) treatment.Methods Ninety one patients with chronic HBV infections admitted in Third Hospital of Hebei Medical University during October 2012 and August 2013 were enrolled in the study.All patients received ADV treatment ( monotherapy or combination therapy) for at least two years, among whom 31 had hypophosphatemia and 60 had normal serum phosphorus levels.rs6420094 and rs4074995 were genotyped by polymerase chain reaction-restrictive fragment length polymorphism ( PCR-RFLP ) analysis.The relationship between allele frequency and serum phosphorus concentration was analyzed by Chi-Square test.Results In hypophosphatemia group, there were 13, 13, and 5 patients with genotype A/A, A/G and G/G at rs6420094, respectively;while in patients with normal serum phosphorus levels, there were 35, 24 and 1 patients with genotype A/A, A/G and G/G at rs6420094.The frequency of allele A at rs6420094 in patients with normal serum level of phosphorus was higher than that in hypophosphatemia group ( 78.3% vs.62.9%, χ2 =4.947, P <0.05 ).In hypophosphatemia group, there were 2, 11, and 18 patients with genotype A/A, A/G and G/G at rs4074995, respectively;while in patients with normal serum phosphorus level, there were 1, 21 and 38 patients with genotype A/A, A/G and G/G at rs6420094.There was no difference in the frequencies of alleles at rs4074995 between two groups (χ2 =0.625, P >0.05).Conclusion The polymorphism of rs6420094 gene may be associated with the serum level of phosphorus in patients with chronic HBV infections following long-term treatment of ADV.
