CAJ | 학술논문

为初步掌握烤烟生育期相关性状的遗传机理，利用由开花相对稍早的品种K326和迟花品种兴烟1号杂交所得的F1植株经过组织培养和染色体加倍技术获得的双单倍体（doubled haploid, DH）群体为作图群体，以AFLP和SSR分子标记为主构建了包含23个连锁群、160个标记的烤烟遗传连锁图谱，其中包括23个AFLP标记、1个SRAP标记和136个SSR标记，图谱总长为1814.1 cM。利用复合区间作图法对烟草生育期开花时间和有效叶数进行了初步的遗传分析，结果表明，共检测到3个主效QTL位点与生育期性状相关，其中2个位点（df1和df2）与开花时间相关，分别位于第1和第11连锁群，表型变异贡献率分别为24.5%和17.3%；第3个位点与有效叶数（npl）相关，分布在第3连锁群上，表型变异贡献率为18.6%，该结果为后续利用高通量分析手段对烟草生育期性状进行精细定位奠定了基础。
위초보장악고연생육기상관성상적유전궤리，이용유개화상대초조적품충K326화지화품충흥연1호잡교소득적F1식주경과조직배양화염색체가배기술획득적쌍단배체（doubled haploid, DH）군체위작도군체，이AFLP화SSR분자표기위주구건료포함23개련쇄군、160개표기적고연유전련쇄도보，기중포괄23개AFLP표기、1개SRAP표기화136개SSR표기，도보총장위1814.1 cM。이용복합구간작도법대연초생육기개화시간화유효협수진행료초보적유전분석，결과표명，공검측도3개주효QTL위점여생육기성상상관，기중2개위점（df1화df2）여개화시간상관，분별위우제1화제11련쇄군，표형변이공헌솔분별위24.5%화17.3%；제3개위점여유효협수（npl）상관，분포재제3련쇄군상，표형변이공헌솔위18.6%，해결과위후속이용고통량분석수단대연초생육기성상진행정세정위전정료기출。
A genetic linkage map was built based on one DH population derived from one F1 in order to understand genetic control of growth period traits including days to flower (df) and number of productive leaves (npl) in flue-cured tobacco. The F1 was obtained by crossing early-flowering cultivar K326 with late-flowering cultivar Xingyan No.1.The map was composed of 23 AFLP, one SRAP and 136 SSR molecular markers assembled into 23 linkage groups (LG1–LG23) with a total genetic distance of 1814.1 cM. Composite interval mapping (CIM) was used to detect QTLs involved in df and npl. Results showed that a total of three main-effect QTLs were detected, of which two (df1 in LG1 and df2 in LG11) were related to df and one (npl in LG3) to npl. Df1 and df2 accounted for 24.5%and 17.3%of phenotypic variation of df, respectively. Npl accounted for 18.6%of npl variation. The primary conclusion can provide good reference for further study in mapping growth period traits in tobacco by using high-throughput technology.