中国烟草学报
中國煙草學報
중국연초학보
ACTA TABACARIA SINICA
2014年
6期
63-69
,共7页
王冬%孙九哲%彭玉富%张小全%李丽华%宋洋洋%杨铁钊
王鼕%孫九哲%彭玉富%張小全%李麗華%宋洋洋%楊鐵釗
왕동%손구철%팽옥부%장소전%리려화%송양양%양철쇠
烤烟%叶面分泌物%西柏三烯二醇%茄酮%品种
烤煙%葉麵分泌物%西柏三烯二醇%茄酮%品種
고연%협면분비물%서백삼희이순%가동%품충
flue-cured tobacco%leaf surface exudates%cembratriene-diols%solanone%varieties
为阐明烟草西柏三烯二醇代谢机理,采用GC-MS测定了8306、12451、云烟87、Y892×8306、豫烟11号、NX232和中烟100,7个烤烟品种(系)中部叶龄60 d叶面分泌物组分,并对其中具有代表性的4个材料的西柏三烯二醇合成关键酶基因CYC-1和CYP71D16进行了荧光定量PCR测定,对生育期叶面分泌物的变化、调制后烟叶茄酮含量和感官质量进行了分析评价。结果表明,7个烤烟品种(系)中部叶叶龄60 d时烟叶的叶面分泌物中,共测定出13种非挥发性化合物,主要成分为西柏三烯二醇;8306具有最高的叶面分泌物和西柏三烯二醇含量,豫烟11号次之,12451最低;CYC-1和CYP71D16在8306中的相对表达量显著高于12451和云烟87,初步说明这两个基因的上调表达促进了西柏三烯二醇的合成。成熟期具有较大西柏三烯二醇降解量的8306和豫烟11号在调制后拥有更高的茄酮含量。
為闡明煙草西柏三烯二醇代謝機理,採用GC-MS測定瞭8306、12451、雲煙87、Y892×8306、豫煙11號、NX232和中煙100,7箇烤煙品種(繫)中部葉齡60 d葉麵分泌物組分,併對其中具有代錶性的4箇材料的西柏三烯二醇閤成關鍵酶基因CYC-1和CYP71D16進行瞭熒光定量PCR測定,對生育期葉麵分泌物的變化、調製後煙葉茄酮含量和感官質量進行瞭分析評價。結果錶明,7箇烤煙品種(繫)中部葉葉齡60 d時煙葉的葉麵分泌物中,共測定齣13種非揮髮性化閤物,主要成分為西柏三烯二醇;8306具有最高的葉麵分泌物和西柏三烯二醇含量,豫煙11號次之,12451最低;CYC-1和CYP71D16在8306中的相對錶達量顯著高于12451和雲煙87,初步說明這兩箇基因的上調錶達促進瞭西柏三烯二醇的閤成。成熟期具有較大西柏三烯二醇降解量的8306和豫煙11號在調製後擁有更高的茄酮含量。
위천명연초서백삼희이순대사궤리,채용GC-MS측정료8306、12451、운연87、Y892×8306、예연11호、NX232화중연100,7개고연품충(계)중부협령60 d협면분비물조분,병대기중구유대표성적4개재료적서백삼희이순합성관건매기인CYC-1화CYP71D16진행료형광정량PCR측정,대생육기협면분비물적변화、조제후연협가동함량화감관질량진행료분석평개。결과표명,7개고연품충(계)중부협협령60 d시연협적협면분비물중,공측정출13충비휘발성화합물,주요성분위서백삼희이순;8306구유최고적협면분비물화서백삼희이순함량,예연11호차지,12451최저;CYC-1화CYP71D16재8306중적상대표체량현저고우12451화운연87,초보설명저량개기인적상조표체촉진료서백삼희이순적합성。성숙기구유교대서백삼희이순강해량적8306화예연11호재조제후옹유경고적가동함량。
Compositions of leaf surface exudates of different tobacco varieties and changes of cembratrien-diols were studied, sensory quality of flue-cured tobacco leaves was also evaluated in order to provide an effective method of finding new tobacco species resources with special aroma. Surface exudates compositions in 60-day-age and mature stage cutters of 7 tobacco varieties, namely 8306, 12451, Yunyan87, Y892×8306, Yuyan No.11, NX232 and Zhongyan100 were determined with GC-MS method. Expression level of CYC-1 and CYP71D16, solanone content and sensory quality in flue-cured tobacco leaves was analyzed. Results showed that 13 flavor substances in compositions of leaf surface exudates in 60-day-age cutters of the 7 tobacco varieties were detected with cembratriene-diols being the main substance. The content of cembratriene-diols, being the main flavor composition, varied among varieties with the highest content in 8306 at 60 days after leaf sprouting, followed by Yuyan No.11 and 12451. Expression level of CYC-1 and CYP71D16 in 8306 was significantly higher than that in 12451 and Yunyan87 while8306 and Yuyan No.11 had higher content of solanone.