CAJ | 학술논문

为阐明烟草西柏三烯二醇代谢机理，采用GC-MS测定了8306、12451、云烟87、Y892×8306、豫烟11号、NX232和中烟100，7个烤烟品种（系）中部叶龄60 d叶面分泌物组分，并对其中具有代表性的4个材料的西柏三烯二醇合成关键酶基因CYC-1和CYP71D16进行了荧光定量PCR测定，对生育期叶面分泌物的变化、调制后烟叶茄酮含量和感官质量进行了分析评价。结果表明，7个烤烟品种（系）中部叶叶龄60 d时烟叶的叶面分泌物中，共测定出13种非挥发性化合物，主要成分为西柏三烯二醇；8306具有最高的叶面分泌物和西柏三烯二醇含量，豫烟11号次之，12451最低；CYC-1和CYP71D16在8306中的相对表达量显著高于12451和云烟87，初步说明这两个基因的上调表达促进了西柏三烯二醇的合成。成熟期具有较大西柏三烯二醇降解量的8306和豫烟11号在调制后拥有更高的茄酮含量。
위천명연초서백삼희이순대사궤리，채용GC-MS측정료8306、12451、운연87、Y892×8306、예연11호、NX232화중연100，7개고연품충（계）중부협령60 d협면분비물조분，병대기중구유대표성적4개재료적서백삼희이순합성관건매기인CYC-1화CYP71D16진행료형광정량PCR측정，대생육기협면분비물적변화、조제후연협가동함량화감관질량진행료분석평개。결과표명，7개고연품충（계）중부협협령60 d시연협적협면분비물중，공측정출13충비휘발성화합물，주요성분위서백삼희이순；8306구유최고적협면분비물화서백삼희이순함량，예연11호차지，12451최저；CYC-1화CYP71D16재8306중적상대표체량현저고우12451화운연87，초보설명저량개기인적상조표체촉진료서백삼희이순적합성。성숙기구유교대서백삼희이순강해량적8306화예연11호재조제후옹유경고적가동함량。
Compositions of leaf surface exudates of different tobacco varieties and changes of cembratrien-diols were studied, sensory quality of flue-cured tobacco leaves was also evaluated in order to provide an effective method of finding new tobacco species resources with special aroma. Surface exudates compositions in 60-day-age and mature stage cutters of 7 tobacco varieties, namely 8306, 12451, Yunyan87, Y892×8306, Yuyan No.11, NX232 and Zhongyan100 were determined with GC-MS method. Expression level of CYC-1 and CYP71D16, solanone content and sensory quality in flue-cured tobacco leaves was analyzed. Results showed that 13 flavor substances in compositions of leaf surface exudates in 60-day-age cutters of the 7 tobacco varieties were detected with cembratriene-diols being the main substance. The content of cembratriene-diols, being the main flavor composition, varied among varieties with the highest content in 8306 at 60 days after leaf sprouting, followed by Yuyan No.11 and 12451. Expression level of CYC-1 and CYP71D16 in 8306 was significantly higher than that in 12451 and Yunyan87 while8306 and Yuyan No.11 had higher content of solanone.