中国烟草科学
中國煙草科學
중국연초과학
CHINESE TOBACCO SCIENCE
2014年
6期
48-53
,共6页
杨珍福%吴毅歆%陈映岚%何月秋
楊珍福%吳毅歆%陳映嵐%何月鞦
양진복%오의흠%진영람%하월추
烟草%黑胫病菌%解淀粉芽孢杆菌%生物防治%促生长
煙草%黑脛病菌%解澱粉芽孢桿菌%生物防治%促生長
연초%흑경병균%해정분아포간균%생물방치%촉생장
tobacco%Phytophthora parasitica var. nicotianae%Bacillus amyloliquefaciens%biocontrol%growth promotion
为了获得能促进烟草生长和防治黑胫病的稳定菌株，通过常规方法从烟草茎、叶中分离到478株内生细菌，其中65株能拮抗烟草黑胫病菌，占13.60%。菌株YN201408、YN201442、YN201448和YN201458对烟草疫霉的抑菌率分别为63.85%、60.82%、64.72%和76.07%，用1.0×107 cfu/mL拮抗菌液浸烟苗根系30 min，移栽后浇灌1次，在温室盆栽试验中控病效果分别为68.24%、65.47%、69.12和41.40%。YN201448对烟草种子萌芽率和烟草的生长都有促进作用，烟草的发芽率、第5~6真叶期苗高和地上部鲜重分别高于清水对照10.95%、29.31%和67.24%。依据菌体形态、生理生化特征和16S rDNA序列，YN201408、YN201442和YN201448鉴定为解淀粉芽孢杆菌，YN201458为铜绿假单胞菌。YN201448因良好的促生长和控病效果而具有继续开发应用的潜力。
為瞭穫得能促進煙草生長和防治黑脛病的穩定菌株，通過常規方法從煙草莖、葉中分離到478株內生細菌，其中65株能拮抗煙草黑脛病菌，佔13.60%。菌株YN201408、YN201442、YN201448和YN201458對煙草疫黴的抑菌率分彆為63.85%、60.82%、64.72%和76.07%，用1.0×107 cfu/mL拮抗菌液浸煙苗根繫30 min，移栽後澆灌1次，在溫室盆栽試驗中控病效果分彆為68.24%、65.47%、69.12和41.40%。YN201448對煙草種子萌芽率和煙草的生長都有促進作用，煙草的髮芽率、第5~6真葉期苗高和地上部鮮重分彆高于清水對照10.95%、29.31%和67.24%。依據菌體形態、生理生化特徵和16S rDNA序列，YN201408、YN201442和YN201448鑒定為解澱粉芽孢桿菌，YN201458為銅綠假單胞菌。YN201448因良好的促生長和控病效果而具有繼續開髮應用的潛力。
위료획득능촉진연초생장화방치흑경병적은정균주，통과상규방법종연초경、협중분리도478주내생세균，기중65주능길항연초흑경병균，점13.60%。균주YN201408、YN201442、YN201448화YN201458대연초역매적억균솔분별위63.85%、60.82%、64.72%화76.07%，용1.0×107 cfu/mL길항균액침연묘근계30 min，이재후요관1차，재온실분재시험중공병효과분별위68.24%、65.47%、69.12화41.40%。YN201448대연초충자맹아솔화연초적생장도유촉진작용，연초적발아솔、제5~6진협기묘고화지상부선중분별고우청수대조10.95%、29.31%화67.24%。의거균체형태、생리생화특정화16S rDNA서렬，YN201408、YN201442화YN201448감정위해정분아포간균，YN201458위동록가단포균。YN201448인량호적촉생장화공병효과이구유계속개발응용적잠력。
To obtain stable bacterial strains with good growth promotion of tobacco and black shrank disease control effect, 478 endophytic bacterial strains were isolated from tobacco leaves and stems. Among them 65 of strains ( accounting for 13.60%) could inhibit Phytophthora parasitica var. nicotianae by the traditional methodology. Especially, the four strains, YN201408, YN201442, YN201448 and YN201458 inhibited 63.85%, 60.82%, 64.72%and 76.07%of mycelia growth of the pathogen and controlled 68.24%, 65.47%, 69.12 and 41.40%of disease severity by soaking the seedling root in 1.0×107cfu/mL of the bacterial suspension for 30 min and drenching once after transplanting in the pot experiment in the greenhouse, respectively. YN201448 could promote seed germination and growth of tobacco, showing 10.95%, 29.31%and 67.24%higher than water control in germination rate, seedling height during 5-6 leaf stage and fresh weight on the ground. YN201408, YN201442 and YN201448 were classified into Bacillus amyloliquefaciens, and YN201458 into Pseudomonas aeruginosa based on their morphology, physiological and biochemical traits and 16S rDNA sequences. YN201448 showed good potential for application based on its growth promotion and disease control effect.
