CAJ | 학술논문

目的：对比评价人血清游离DNA的分支DNA（bDNA）检测和实时荧光定量聚合酶链反应（RT-qPCR）检测方法。方法：收集44例人血清标本，分别运用bDNA技术和荧光定量PCR检测其游离DNA浓度，对2种方法检测结果做分析比较。同时采用bDNA技术分别检测其中10例血清和血浆游离DNA含量。结果：bDNA技术的检测结果和荧光定量PCR具有较高的相关性（r=0.7077，P<0.0001）；血清较血浆具有较高的游离DNA的水平。结论：bDNA操作简便且结果可信，适用于人群大规模筛查游离DNA水平。
목적：대비평개인혈청유리DNA적분지DNA（bDNA）검측화실시형광정량취합매련반응（RT-qPCR）검측방법。방법：수집44례인혈청표본，분별운용bDNA기술화형광정량PCR검측기유리DNA농도，대2충방법검측결과주분석비교。동시채용bDNA기술분별검측기중10례혈청화혈장유리DNA함량。결과：bDNA기술적검측결과화형광정량PCR구유교고적상관성（r=0.7077，P<0.0001）；혈청교혈장구유교고적유리DNA적수평。결론：bDNA조작간편차결과가신，괄용우인군대규모사사유리DNA수평。
Objective:Compare two methods to detect the concentrations of serum cell-free DNA by using branched DNA assay (bDNA) and real-time fluorescence quantitative real-time polymerase chain reaction (RT-qPCR). Methods: 44 samples of serum cell-free DNA were measured by both bDNA based on Alu sequence and qPCR. The correlation between the two methods was analyzed by a Spearman rank test. Detection of cell-free DNA in human plasma and serum was ana_lyzed by bDNA. Results: It was demonstrated in the present study that the bDNA method was highly correlated with real-time PCR (r=0.7077, P<0.0001). Cell-free DNA concentrations were higher in serum than in plasma. Conclusion: bDNA assay had advantages of lower time and labor compared to the qPCR method that is applicable to screening consideration.