中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
CHINESE JOURNAL OF MATERNAL AND CHILD HEALTH RESEARCH
2014年
6期
978-981
,共4页
缺氧诱导因子%妊娠高血压疾病%胎盘滋养细胞%表达差异
缺氧誘導因子%妊娠高血壓疾病%胎盤滋養細胞%錶達差異
결양유도인자%임신고혈압질병%태반자양세포%표체차이
hypoxia-inducible factor%pregnancy-induced hypertension%placental trophoblast cells%expression difference
目的：分析缺氧诱导因子（ HIF）在妊娠高血压疾病患者胎盘滋养细胞中的表达差异，评价其临床价值。方法选取重庆市沙坪坝区人民医院2013年1月至2013年12月分娩孕妇170例，分为正常对照组50例，妊娠高血压疾病组85例，轻度子痫前期组22例，重度子痫前期组13例。应用免疫组织化学方法及Western blot法，比较各组胎盘组织HIF-1α的表达和HIF-1α／β-actin的相对吸光度比值；免疫组织化学方法比较各组胎盘组织中HIF-2蛋白的表达，采用RT-PCR方法检测HIF-2αmRNA水平。结果妊娠高血压疾病组、轻度子痫前期组和重度子痫前期组的HIF-1α和HIF-1α／β-actin的相对吸光度比值均显著高于正常组（ t值分别为11．776、17．216、21．168、9．979、13．171、21．406，均P＜0．01）。重度子痫前期组的HIF-1α和HIF-1α／β-actin的相对吸光度比值显著高于轻度子痫前期组和妊娠高血压疾病组（t值分别为7．372、7．449、11．623、23．338，均P＜0．01）。妊娠高血压疾病组、轻度子痫前期组和重度子痫前期组的HIF-2αmRNA及蛋白均显著高于正常组（ t值分别为17．344、29．253、34．833、39．388、41．850、38．567，均P＜0．01）；重度子痫组的HIF-2αmRNA及蛋白显著高于轻度子痫前期组和妊娠高血压疾病组（ t值分别为6．557、6．348、30．263、19．314，均P＜0．01）。 HIF-1α在妊娠高血压疾病组、轻度子痫前期组和重度子痫前期组中的表达率均显著高于正常组（χ2值分别为8．434、9．680、4．225，均P＜0．05），HIF-1α在轻度子痫前期组的表达率显著高于妊娠高血压疾病组（χ2＝4．919，P＜0．05），HIF-1α在轻度子痫前期组的表达率和重度子痫前期组无显著差异（χ2＝0．000，P＞0．05）。结论缺氧诱导因子HIF-1α和HIF-2α在妊娠期高血压疾病中具有重要意义。
目的：分析缺氧誘導因子（ HIF）在妊娠高血壓疾病患者胎盤滋養細胞中的錶達差異，評價其臨床價值。方法選取重慶市沙坪壩區人民醫院2013年1月至2013年12月分娩孕婦170例，分為正常對照組50例，妊娠高血壓疾病組85例，輕度子癇前期組22例，重度子癇前期組13例。應用免疫組織化學方法及Western blot法，比較各組胎盤組織HIF-1α的錶達和HIF-1α／β-actin的相對吸光度比值；免疫組織化學方法比較各組胎盤組織中HIF-2蛋白的錶達，採用RT-PCR方法檢測HIF-2αmRNA水平。結果妊娠高血壓疾病組、輕度子癇前期組和重度子癇前期組的HIF-1α和HIF-1α／β-actin的相對吸光度比值均顯著高于正常組（ t值分彆為11．776、17．216、21．168、9．979、13．171、21．406，均P＜0．01）。重度子癇前期組的HIF-1α和HIF-1α／β-actin的相對吸光度比值顯著高于輕度子癇前期組和妊娠高血壓疾病組（t值分彆為7．372、7．449、11．623、23．338，均P＜0．01）。妊娠高血壓疾病組、輕度子癇前期組和重度子癇前期組的HIF-2αmRNA及蛋白均顯著高于正常組（ t值分彆為17．344、29．253、34．833、39．388、41．850、38．567，均P＜0．01）；重度子癇組的HIF-2αmRNA及蛋白顯著高于輕度子癇前期組和妊娠高血壓疾病組（ t值分彆為6．557、6．348、30．263、19．314，均P＜0．01）。 HIF-1α在妊娠高血壓疾病組、輕度子癇前期組和重度子癇前期組中的錶達率均顯著高于正常組（χ2值分彆為8．434、9．680、4．225，均P＜0．05），HIF-1α在輕度子癇前期組的錶達率顯著高于妊娠高血壓疾病組（χ2＝4．919，P＜0．05），HIF-1α在輕度子癇前期組的錶達率和重度子癇前期組無顯著差異（χ2＝0．000，P＞0．05）。結論缺氧誘導因子HIF-1α和HIF-2α在妊娠期高血壓疾病中具有重要意義。
목적：분석결양유도인자（ HIF）재임신고혈압질병환자태반자양세포중적표체차이，평개기림상개치。방법선취중경시사평패구인민의원2013년1월지2013년12월분면잉부170례，분위정상대조조50례，임신고혈압질병조85례，경도자간전기조22례，중도자간전기조13례。응용면역조직화학방법급Western blot법，비교각조태반조직HIF-1α적표체화HIF-1α／β-actin적상대흡광도비치；면역조직화학방법비교각조태반조직중HIF-2단백적표체，채용RT-PCR방법검측HIF-2αmRNA수평。결과임신고혈압질병조、경도자간전기조화중도자간전기조적HIF-1α화HIF-1α／β-actin적상대흡광도비치균현저고우정상조（ t치분별위11．776、17．216、21．168、9．979、13．171、21．406，균P＜0．01）。중도자간전기조적HIF-1α화HIF-1α／β-actin적상대흡광도비치현저고우경도자간전기조화임신고혈압질병조（t치분별위7．372、7．449、11．623、23．338，균P＜0．01）。임신고혈압질병조、경도자간전기조화중도자간전기조적HIF-2αmRNA급단백균현저고우정상조（ t치분별위17．344、29．253、34．833、39．388、41．850、38．567，균P＜0．01）；중도자간조적HIF-2αmRNA급단백현저고우경도자간전기조화임신고혈압질병조（ t치분별위6．557、6．348、30．263、19．314，균P＜0．01）。 HIF-1α재임신고혈압질병조、경도자간전기조화중도자간전기조중적표체솔균현저고우정상조（χ2치분별위8．434、9．680、4．225，균P＜0．05），HIF-1α재경도자간전기조적표체솔현저고우임신고혈압질병조（χ2＝4．919，P＜0．05），HIF-1α재경도자간전기조적표체솔화중도자간전기조무현저차이（χ2＝0．000，P＞0．05）。결론결양유도인자HIF-1α화HIF-2α재임신기고혈압질병중구유중요의의。
Objective To analyze the expression changes and clinical value of hypoxia-inducible factor in placental trophoblast cells with pregnancy-induced hypertension .Methods From January 2013 to December 2013 170 cases of pregnant women giving birth in Chongqing Municipal People ’ s Hospital of Shapingba District were divided into normal control group ( 50 cases ) , pregnancy-induced hypertension group (85 cases), mild preeclampsia group (22 cases) and severe preeclampsia group (13 cases).Immunohistochemical methods and Western blot method were used to compare the expression of HIF-1αand HIF-1α/β-actin relative absorbance ratio in each group .The expression of HIF-2 protein was compared among different groups by immunohistochemical methods , and HIF-2αmRNA level was detected by RT-PCR method.Results The expression of HIF-1αand relative absorbance ratio of HIF-1α/β-actin of pregnancy-induced hypertension group, mild preeclampsia group and severe preeclampsia group were significantly higher than those of the normal group ( t value was 11.776, 17.216, 21.168, 9.979, 13.171 and 21.406, respectively, all P<0.01).In severe preeclampsia group the expression of HIF-1αand HIF-1α/β-actin relative absorbance ratio were significantly higher than those in mild preeclampsia group and pregnancy -induced hypertension group (t value was 7.372, 7.449, 11.623 and 23.338, respectively, all P<0.01).In pregnancy-induced hypertension group, mild preeclampsia group and severe preeclampsia group HIF-2αmRNA and protein were significantly higher than those in the normal group (t value was 17.344, 29.253, 34.833, 39.388, 41.850 and 38.567, respectively, all P<0.01).HIF-2αmRNA and protein in severe preeclampsia group were significantly higher than those in mild preeclampsia group and pregnancy -induced hypertension group (t value was 6.557, 6.348, 30.263 and 19.314, respectively, all P <0.01).HIF-1αexpression in pregnancy-induced hypertension group , mild preeclampsia group and severe preeclampsia group was significantly higher than that in normal group (χ2 value was 8.434, 9.680 and 4.225, respectively, all P<0.05), and it was higher in mild preeclampsia group than in pregnancy-induced hypertension group (χ2 =4.919,P<0.05).There was no significant difference in HIF-1αexpression between mild preeclampsia group and severe preeclampsia group (χ2 =0.000, P>0.05).Conclusion The hypoxia-inducible factors HIF-1αand HIF-2αhave significance in pregnancy-induced hypertension .
