CAJ | 학술논문

以水稻品种“东稻4”与“吉粳88”为供试材料，分别种植在吉林西部轻度与重度苏打盐碱地中，在花药发育不同时期进行取样，利用石蜡切片与扫描电镜2种方法观察其花药发育不同时期的小孢子、花粉粒、绒毡层形态上的变化以及成熟花粉粒表面结构的变化。结果表明：苏打盐碱胁迫对2个水稻品种花药发育的影响均表现为小孢子、花粉粒形态上出现萎蔫，细胞质出现皱缩；绒毡层不降解或非正常降解；成熟花粉粒萌发孔环与萌发孔盖结构出现萎缩。轻度与重度苏打盐碱胁迫下“吉粳88”花药发育各个时期小孢子、花粉粒萎蔫程度，细胞质皱缩程度均大于“东稻4”；“吉粳88”在重度苏打盐碱胁迫下观察到花粉粒败育与绒毡层脱落，而“东稻4”未发现；重度苏打盐碱胁迫下“吉粳88”成熟花粉粒萌发孔环、萌发孔盖萎缩程度大于“东稻4”。通过上述结果对比得知，“东稻4”的耐盐碱性可能强于“吉粳88”。
이수도품충“동도4”여“길갱88”위공시재료，분별충식재길림서부경도여중도소타염감지중，재화약발육불동시기진행취양，이용석사절편여소묘전경2충방법관찰기화약발육불동시기적소포자、화분립、융전층형태상적변화이급성숙화분립표면결구적변화。결과표명：소타염감협박대2개수도품충화약발육적영향균표현위소포자、화분립형태상출현위언，세포질출현추축；융전층불강해혹비정상강해；성숙화분립맹발공배여맹발공개결구출현위축。경도여중도소타염감협박하“길갱88”화약발육각개시기소포자、화분립위언정도，세포질추축정도균대우“동도4”；“길갱88”재중도소타염감협박하관찰도화분립패육여융전층탈락，이“동도4”미발현；중도소타염감협박하“길갱88”성숙화분립맹발공배、맹발공개위축정도대우“동도4”。통과상술결과대비득지，“동도4”적내염감성가능강우“길갱88”。
Taking rice varieties dongdao 4 and jijing 88 as test materials, paraffinsection and SEM as analysis methods, we studied the changes of microspore, pollen, tapetum and surface structure of mature pollen grains of the two varieties at different pollen development stages under typical slight and severe saline?sodic soils in western Jilin province. It turned out that both dongdao 4 and jijing 88 showed similar symptoms under soda?sodic stress:rice microspore and pollen wilted, and pollen pro?toplasm shrank;tapetum structural layers showed abnormal degradation or no degradation;germinal grommet and germinal lid of mature pollen gradually shrank. The wilting degree of microspore and pollen at different pollen development stages of jijing 88 was more serious than dongdao 4, so the pollen protoplasm did. Additionally, under the severe saline?sodic soil, jijing 88 showed sterile pol?lens and the structural layers of tapetum disintegrated, but dongdao 4 didn′t. The shrinking of ma?ture pollen germinal grommet and germinal lid of jijing 88 was also more obvious than dongdao 4. According to the above comparison, the saline?alkaline resistance of dongdao 4 may be stronger than jijing 88.